金黄色葡萄球菌分离株致病基因研究  

Study of virulence genes in Staphylococcus aureus isolates

作　　者：岳丽琴 王俊怡 徐小静 李向梅[2] 耿文静[2] 吴德静[2] 沈叙庄[2] 

机构地区：[1]清华大学第一附属医院,北京100045 [2]首都医科大学附属北京儿童医院微生物及免疫学实验室,北京100016

出　　处：《临床儿科杂志》2013年第4期347-350,共4页Journal of Clinical Pediatrics

摘　　要：目的研究金黄色葡萄球菌致病基因在金黄色葡萄球菌分离株中的分布。方法用PCR方法对分离自肺炎患儿的60株金黄色葡萄球菌进行19种超抗原基因、2种剥脱毒素基因和3种黏附基因进行检测。结果 60株金黄色葡萄球菌,最常见的为sek(50%,30/60),其次为seq(48%,29/60)、sea(38%,23/60)、seb(37%,22/60)。未检测到sed、etd、sep。3种黏附基因bbp、sdrE、cna分别存在于87%、65%、40%的菌株中。结论分离自肺炎患儿的金黄色葡萄球菌sek超抗原基因有较高的流行性,3种黏附基因的携带率不同于其他地区,致病基因的作用尚需进一步的研究。Objective To study virulence genes of Staphylococcus aureus （S. aureus） isolates from Chinese pediatric patients. Methods A total of 60 clinical S. aureus isolates were collected from pediatric patients with pneumonia and were tested by PCR for 19 superantigen genes, 3 adhesin and 2 toxin genes. Results Among the 60 clinical isolates, sek（50%, 30/60） was the most common superantigen gene, followed by seq （29%, 48/60）, sea （38%, 23/60） and seb （37%, 22/60）, but sed, etd, and sep genes were not detected. Three adhesin genes, bbp, sdrE and cna were found in 87%, 65% and 40% of clinical isolates, respectively. Conclusions The sek was the most common superantigen gene ofS. aureus isolated from pediatric patients with pneumonia. There is regional difference in the distribution of 3 adhesin genes. The role of virulence genes requires further study.

关 键 词：金黄色葡萄球菌 致病基因 儿童 

分 类 号：R378[医药卫生—病原生物学]