γ-干扰素抗体对早期流产大鼠γ-干扰素、白细胞介素-2、白细胞介素-4的表达及滋养层细胞凋亡的影响  

Effects of IFN-γ antibody on the expression of IFN-γ, IL-2, IL-4 and apoptosis of trophoblast in early abortion rat

作　　者：赵伟[1] 刘冉冉[1] 王东[1] 李雅娜[1] 张连双[1] 王跃嗣[2] 

机构地区：[1]滨州医学院组织学与胚胎学教研室 [2]滨州医学院生物技术教研室,烟台264003

出　　处：《解剖学杂志》2013年第2期148-151,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金（Y2007C167）

摘　　要：目的：探讨γ干扰素（IFN-γ）抗体对孕鼠早期流产的影响及其机制。方法：实验孕鼠随机分成流产模型组、IFN-γ抗体组、流产模型＋IFN-γ抗体组及正常对照组。酶联免疫吸附法测定各组大鼠血清IFN-γ、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）水平，免疫组织化学检测子宫蜕膜组织中Bcl-2和Bax蛋白的表达，Hoechst33342荧光染色检测滋养层细胞凋亡情况。结果：血清测定数据显示，流产模型组IFN-7、IL-2水平高于正常对照组，IL-4水平低于正常对照组；流产模型＋IFN-7抗体组IFN-γ、IL-2水平低于流产模型组，高于IFN-γ抗体组；IL-4水平高于流产模型组，低于IFN-γ抗体组。免疫组织化学Bcl-2和Bax蛋白阳性产物光密度值比值（Bax／Bcl-2）流产模型＋IFN-γ抗体组低于流产模型组。结论：大鼠血清中3种细胞因子IFN-γ、IL-2、IL-4表达的改变及滋养层细胞凋亡可能是流产发生的重要因素。应用IFN-γ抗体可降低孕鼠IFN-γ、IL-2水平，升高IL-4水平，抑制滋养层细胞的凋亡，从而使流产率降低。Objective： To investigate the effect and mechanism of IFN-γ antibody on pregnant rats in early abortion. Methods： Pregnant rats were randomly divided into abortion model group, IFN-γ antibody group, abortion model ＋IFN-γ antibody group and normal control group. On the 9th day, enzyme linked immunosorbent assay was used to assay the concentration of IFN-γ, IL-2 and IL-4 in blood serum. Immunohistochemistry was used to detect the expression of Bcl-2 and Bax protein in de- cidua of uterus. Hoechst 33342 fluorescence staining was used to detect apoptosis in trophoblast. Results： The serum IFN-γ and IL-2 levels of the model group were higher than those in the control group, and IL-4 level was lower than that in the nor mal control group~ The serum IFN-γand IL-2 levels of the abortion model＋IFN-γ antibody group were lower than those in the model group, and higher than those in the IFN-γ antibody group; and IL-4 level was higher than that in the model group, and lower than that in the IFN-γ antibody group. The OD value ratio of immunohistochemistry positive products of Bax and Bcl-2 protein （Bax/Bcl-2） of the abortion model＋IFN-γ, antibody group was lower than that in the model group. The changes of apoptosis detected by Hoechst 33342 fluorescence staining and the results detected by immunohistochemistry showed a simi lar tendency. Conclusion.. The expression change of IFN-γ, IL-2 and IL-4 in serum and the apoptosis of trophoblast may be important to the mechanism of abortion. Application of IFN-γ antibody can reduce the level of IFN-γ and IL-2, elevate the level of IL-4, and inhibit the apoptosis of trophocyte, thereby it can debase the rate of abortion.

关 键 词：γ干扰素 白细胞介素-2 白细胞介素-4 细胞凋亡 流产 

分 类 号：R730.53[医药卫生—肿瘤]