CCK-8对癫痫发作大鼠脑内SOD和MDA的影响  

THE EFFECTS OF CCK-8 ON SOD AND MDA DURING EPILEPSY SEIZURE

作　　者：张洪燕[1] 徐珞[1] 陶尚敏[1] 

机构地区：[1]青岛大学医学院生理教研室,青岛266021

出　　处：《中国应用生理学杂志》2000年第3期206-209,共4页Chinese Journal of Applied Physiology

基　　金：山东省教育委员会基金!资助课题 (J95K34)

摘　　要：目的和方法 :采用核团微量注射、光化学分析等实验方法 ,观察大鼠脑内SOD和MDA在CCK 8调节癫痫发作中的变化。结果 :①与正常大鼠比较 ,遗传性听源性癫痫易感大鼠皮层、海马、下丘脑及垂体内SOD活性、MDA含量无显著差异 (P >0 .0 5 ) ;②大鼠癫痫发作后 ,上述区域内SOD活性明显降低 (P <0 .0 5 ) ,而MDA含量明显增加 (P <0 .0 5 ) ,若癫痫发作次数增加 ,该变化愈显著 (P <0 .0 1) ;③大鼠海马CA3 区注射CCK 8后 ,皮层、海马、下丘脑及垂体内SOD活性及MDA含量无明显改变 (P >0 .0 5 )。诱发癫痫时 ,在癫痫发作减弱的同时 ,上述区域内SOD活性显著增高 (P <0 .0 1) ,MDA含量显著降低 (P <0 .0 5 )。结论 :癫痫发作后 ,脑内神经元抗氧化能力降低 ,CCK 8可压抑癫痫发作 ,提高脑内神经元的抗氧化能力。Aim and Methods: The effect of CCK-8 in hippocampus on SOD and MDA during seizure was studied by means of microinjection of CCK-8 and photochemical analysis in rats. Results:①Compared with wistar rats, the content of MDA and SOD activity in the brain were no change in P77PMC rats ( P >0.05). ②After seizure, SOD activity was significantly decreased( P <0.05) and the content of MDA was significantly increased( P <0.05)in the brain. Following the times of seizure increased, the increase of MDA and decrease of SOD changed more and more ( P <0.01).③ After injection of CCK-8 into hippocampus CA 3 area, the content of MDA and SOD activity were no change ( P >0.05); After injection of CCK-8 into hippocampus CA 3 area and inducing seizure, seizure became decreased and SOD activity was significantly increased ( P <0. 01) and the content of MDA was significantly decreased ( P <0.05) in the brain. Conclusion:After seizure the antioxidation ability of neurons in brain was decreased. CCK-8 could inhibit seizure and so enhanced the autioxidation ability of neurons in brain.

关 键 词：超氧化物歧化酶 丙二醛 CCK-8 癫痫 MDA 

分 类 号：R742.1[医药卫生—神经病学与精神病学]