经脂多糖(LPS)处理免疫应答大鼠离体脑片视上核神经元对细胞因子敏感性的变化  

Alteration of sensitivity of supraoptic nucleus neurons to cytokine in the hypothalamic slices from the rat after lipopolysaccharide injection

作　　者：王晓斌[1] 胡三觉[1] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032

出　　处：《生理学报》2000年第4期339-342,共4页Acta Physiologica Sinica

摘　　要：实验采用离体脑片全细胞膜片箝记录方法 ,观察了细胞因子白介素 1β(IL 1β)和IL 2对大鼠离体脑片视上核神经元膜电位及自发放电的影响 ,以期探明免疫应答大鼠视上核神经元对细胞因子敏感性的变化。结果显示 ,用 10 0U/mlIL 1β灌流脑片 ,正常对照的 (n =15 )和脂多糖 (lipopolysaccharideLPS)腹腔注射 9d的大鼠视上核神经元 (n =2 0 )超极化 ,同时伴有自发放电频率的下降 ;应用 10 0U/mlIL 2 ,大部分正常对照视上核神经元 (n =14)表现为超极化 ,自发放电减少 ,剩余部分 (n =3)变化不明显 ;在LPS免疫 9d大鼠离体脑片上的 45个视上核神经元中 ,10 0U/ml的IL 2使其中 19个表现为去极化并伴有自发放电频率增加 ,16个变化不明显 ,其余 10个表现为超极化伴放电频率下降。以上结果表明 ,在免疫应答中 ,视上核神经元对细胞因子IL 2的敏感性 ,在一定的程度上发生了改变 ,细胞因子IL 2可能参与了视上核神经元的功能调节 ,进而在免疫应答过程中发挥了调节作用。Using the whole cell patch clamp technique, the effects of interleukin 1β (IL 1β) and IL 2 on the membrane potential and spontaneous discharges in the supraoptic nucleus (SON) neurons from the rat hypothalamic slices in vitro were examined to determine the changes in sensitivity of supraoptic nucleus neurons to cytokine. The results obtained are as follows. IL 1β (100 U/ml) hyperpolarized the SON neurons from the control rats ( n =15) and the rats 9 d after i p lipopolysaccharide (LPS) ( n =20), and decreased the rate of their spontaneous discharges. When 100 U/ml IL 2 was perfused, most SON neurons ( n =14) from the control rats were hyperpolarized and the spontaneous firing decreased, but the rest ( n =3) remained unchanged. When IL 2 (100 U/ml) was applied, 19 neurons out of the 45 SON neurons from the rats 9 days after i p LPS were depolarized accompanied with an increase of the discharges, 16 did not show significant changes in both membrane potential and spontaneous discharges, the rest 10 were hyperpolarized and their discharges were suppressed. These results show that the sensitivity of part of SON neurons from the immuned rat to cytokine IL 2 was altered, suggesting that IL 2 may be involved in modulation of the activity of SON neurons and thus play a role in modulation of the immune response.

关 键 词：视上核 LPS 

分 类 号：R338.2[医药卫生—人体生理学]