农杆菌介导DR1172基因转化甘蓝型油菜的研究  

On Agrobacterium-mediated Transformation of DR1172 Genes into Brasscia napus L.

作　　者：张新[1] 崔广艳[1] 陈翠娜[1] 张思维[1] 代其林[1] 王劲[2] 

机构地区：[1]西南科技大学极端环境生物资源利用实验室,四川绵阳621000 [2]中国农业科学院生物技术研究所,北京100084

出　　处：《绵阳师范学院学报》2013年第2期51-56,共6页Journal of Mianyang Teachers＇ College

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅(09ZA034);西南科技大学博士研究基金(11zx7104)

摘　　要：耐辐射奇球菌(Deinococcus.Radiodurans,DR)能在极端胁迫条件下生存,并且拥有一个独特的极端环境抗性基因组而被广泛用于研究.其中DR1172是在耐辐射球菌中分离出的一段基因,与LEA76家族同源,参与植物的抗旱机制.本研究利用基因工程手段以载体pBI121为基础构建了植物DR1172-EHA105表达载体,然后利用农杆菌介导法将目的基因DR1172转入油菜中,得到再生油菜植株97株,阳性植株48棵,转化植株阳性率为49.48%.该研究为探讨DR1172基因的功能以及植物抗逆性提供了丰富的植物材料,而且对利用生物工程技术手段改良植物性状,培育出抗逆性强的植物优良品种有重要的指导价值.DR1172 is a part of gene from Deinococcus Radiodurans which has high resistance to radiation resistance. DR1172 and LEA76 are actually homologous in some way that they both participate in the drought resist- ant in plants. In this research, the expression vector DRl172 - EHA105 was constructed successfully and the DR1172 genes were inserted into Brassica napus L. by using the transgenic technique. Transgenic Brescia napus L. were identified by the molecular identification PCR. 48 positive Brassica napus L. were got. The researches not only provide abundant plant resources to study the function of DR1172, but also have great important application value in plant gene engineering technology and cultivate quality variety of plants and agricultural production.

关 键 词：DR1172基因 载体构建 基因工程 油菜 

分 类 号：Q785[生物学—分子生物学]