脉冲电场对水稻纹枯菌的杀灭效果  

Effect of pulsed electric field on Rhizoctorzia solani

作　　者：冯学斌[1] 尹文庆[1] 王迎迎[1] 张美娜[1] 

机构地区：[1]南京农业大学工学院,江苏南京210031

出　　处：《浙江农业学报》2013年第2期365-369,共5页Acta Agriculturae Zhejiangensis

摘　　要：探讨了脉冲电场杀菌的机理,仿真分析了培养液中脉冲电场的分布情况,仿真结果表明:进入培养液内脉冲电场时域波形的脉宽都有不同程度的变窄;脉冲上升沿越陡峭,进入培养液内部的脉冲电场峰值越高,穿透性越好。对脉冲电场水稻纹枯菌杀灭效果进行了试验研究,在同轴型处理室中分别以不同幅值的电磁脉冲(频率为2.5Hz)和不同的作用时间对10-4稀释的水稻纹枯菌菌液进行处理,试验结果表明:当电磁脉冲的幅值较低时,可以通过延长灭菌的时间来提高灭菌效果;而幅值较高时,则脉冲幅值为灭菌效果的主要影响因素,延长灭菌时间的意义不大。Principle of pulse electric field （PEF） pasteurization was discussed and the distribution of the PEF in cul- ture fluid was analyzed through simulation, the simulation results showed that pulse widths of the PEF time domain waveform in culture fluid were narrowed at different degrees. Peak of the PEF was higher and penetrating was better when the input pulse rising edge was steeper. Effects of PEF sterilization on Rhizoctorzia solani were studied, liquid of Rhizoctorzia solani diluted to 10 -4 were processed in the coaxial treatment chamber with different amplitudes of the electromagnetic pulse （ EMP, a frequency of 2. 5 Hz） and different action duration. The experiments showed that PEF pasteurizationeffect could be enhanced by extending the work time when the pulse amplitude was lower; when the amplitude was higher, the main influence factor of PEF pasteurization was the pulse amplitude while it was not very meaningful to extend the work time.

关 键 词：脉冲电场 水稻纹枯菌 杀菌效果 残活率 

分 类 号：S477[农业科学—农业昆虫与害虫防治]