三角帆蚌蛋白多糖基本特征(英文)  

Preliminary Characterization of Proteoglycan from Hyriopsis cumingii

作　　者：乔德亮[1,2] 王琳[2] 刘俊[2] 孙怡[2] 叶红[2] 曾晓雄[2] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《中药材》2013年第1期15-22,共8页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金研究项目(31271853)

摘　　要：目的:研究三角帆蚌蛋白多糖的基本特征。方法:运用分光光度比色法测量多糖和蛋白质含量,傅立叶变换红外光谱分析官能基团,高效液相色谱法测量相对分子质量和氨基酸组成,气相色谱测量单糖组成,β-消除反应测量糖肽键连接方式。结果:三角帆蚌蛋白多糖中,多糖和蛋白质的含量分别是80.06%和9.42%,傅立叶变换红外光谱显示多糖和蛋白质的特征吸收峰,蛋白多糖的相对分子质量为503.1 kDa,多糖由鼠李糖、岩藻糖、甘露糖、葡萄糖和半乳糖(分子摩尔比为13.80∶4.51∶7.70∶64.92∶9.07)组成,蛋白质部分检测出14种氨基酸(13种已知和1种未知),肽链与多糖链的连接方式为O-型糖肽键。结论:三角帆蚌蛋白多糖的基本特征已初步了解。Objective： To study the preliminary characterizations of Hyriopsis cumingii proteoglycan（HCPG）.Methods： The content of carbohydrate and protein were measured by spectrophotometry.FTIR spectrum was used to analyze the functional groups.Relative molecular mass and amino acid composition were detected by HPLC.GC was utilized to determine the monosaccharide composition.The glycopeptide linkage-bond was detected by using the method of β-elimination reaction.Results： In HCPG,the content of carbohydrate and protein was 80.06% and 9.42%,respectively.FTIR spectrum showed the characteristic absorptions of polysaccharides and protein.Relative molecular mass of HCPG,determined by size-exclusive HPLC,was 503.1 kDa.GC spectra demonstrated that polysaccharide of HCPG was composed of rhamnose,fucose,mannose,glucose and galactose with a molar ratio of 13.80∶ 4.51∶ 7.70∶ 64.92 ∶ 9.07.Fourteen amino acids（13 known and one unknown） have been detected by pre-column derivation HPLC.From β-elimination reaction,peptide chain was attached to the carbohydrate chain by O-glycosidic bond.Conclusion： Basic characterizations of HCPG have been determined preliminarily.

关 键 词：三角帆蚌 蛋白多糖 特征 

分 类 号：R282.6[医药卫生—中药学]