中国内蒙古通辽地区和吉林长春地区宫颈癌中HPV16型E6基因变异分析  

Genetic Variants of HPV16 E6 in Cervical Cancer from Tongliao Area in Inner Mongolia and Changchun City in Jilin Province of China

作　　者：乌恩奇[1] 刘微[2] 王金花[3] 冯丽华[4] 查晓[5] 孔维[2] 

机构地区：[1]中央民族大学中国少数民族传统医学国家民委-教育部重点实验室,北京100081 [2]吉林大学生命科学学院,长春130023 [3]内蒙古民族大学附属医院病理科,内蒙古通辽029100 [4]吉林大学第一医院妇产科,长春130021 [5]四川省肿瘤医院,成都610041

出　　处：《内蒙古大学学报（自然科学版）》2013年第2期157-164,共8页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20674029)

摘　　要：为了解我国内蒙古通辽和吉林长春地区宫颈癌中HPV16亚型的分布情况,对通辽和长春的30例HPV16阳性宫颈癌样品中的HPV16 E6基因进行序列分析.结果在30例宫颈癌样品中的E6全长基因内发现11处位点突变,形成了14种突变体,其中突变T459G是本试验第一次发现的突变.经系统进化分析发现,所有突变体均属欧洲原型突变群.结合之前我国HPV16亚型分布调查结果表明,欧洲型和东亚型HPV16在我国不同地区,分布不均一,主要以欧洲原型突变群为主.To investigate the prevalence of the HPV16 subtypes in Tongliao area in Inner Mongolia and Changchun city in Jilin province in China, the sequences of HPV16 E6 gene were amplified and sequenced from 30 HPV 16 positive cervical cancer samples isolated from Tongliao area in Inner Mongolia and Chang-chun city. A total of 14 variants were detected from 11 nueleotide sites in HPV16 E6 gene in 30 cervical cancer samples. The nucleotide variant T459G has not been reported previously. Phylogenetie analysis showed that all variants detected in this study belong to HPV16 E-Class. Together with the data from previ- ous HPV16 subtype studies in China, the results indicate that HPV16 E-Clas is the most predominant in China, European subtype and East-Asian subtype distributed unregularly in different areas in China.

关 键 词：HPV16 宫颈癌 E6基因 突变 

分 类 号：Q319[生物学—遗传学] R181[医药卫生—流行病学]