RNA干扰及其在植物研究中的应用  

Technology of RNAi and Its Application in Plant Research

作　　者：王婷婷[1] 王丹丹[1] Rahman LaibiChelab 康丹[1] 游腾飞[1] 眭安平[2] 杨星勇[1] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]西南大学图书馆,重庆400715

出　　处：《生物技术通报》2013年第3期48-52,共5页Biotechnology Bulletin

摘　　要：RNA干扰(RNAi)是利用双链RNA特异性地降解同源靶标mRNA,从而特异性地阻断相应基因表达的现象,它属于转录后水平的基因沉默(PTGS)机制。RNAi广泛存在于生物体中,是2l世纪的研究热点之一。近年来,RNAi在植物功能基因组学研究中发挥着越来越重要的作用。在此对RNAi的作用机制和特点,及其在植物基因功能分析、作物品种改良、抗病虫害和抗逆性等方面的研究进行概述,旨在为RNAi在植物研究上的应用提供参考。RNA interference （ RNAi ） belongs to the post-transcriptional gene silencing phenomenon in which double-stranded RNA triggers the degradation of homologous mRNA, thereby specific to suppression the corresponding gene expression. RNAi is a phenomenon that commonly existed in organisms, and it has become one of the hotspots of scientific research in 21＂ century. In recent years, RNAi plays a more and more important role in plant functional genomics. We reviewed characteristics and action mechanism of RNAi, as well as the research progress in aspects including the plant gene function analysis, the crop variety improvement and the resistance to disease or pests, in order to provide reference for RNAi application in plant research.

关 键 词：RNA干扰 双链RNA 基因沉默 植物 应用 

分 类 号：Q943.2[生物学—植物学]