48个油橄榄品种的遗传多样性及聚类分析  

Genetic Diversity and Clustering Analysis on 48 Olive Cultivars

作　　者：陈海云[1] 陈少瑜[1] 宁德鲁[2] 李瑞[1] 李勇杰[2] 毛云玲[1] 吴涛[1] 

机构地区：[1]云南省林业科学院云南省森林植物培育与开发利用重点实验室国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,昆明650204 [2]云南省林业科学院,昆明650204

出　　处：《生物技术通报》2013年第3期96-101,共6页Biotechnology Bulletin

基　　金：云南省重点新产品开发计划项目(2009BB006)

摘　　要：采用ISSR分子标记技术对48个引种和选育的油橄榄品种进行遗传多样性和聚类分析。11条筛选出的引物共扩增出106条DNA谱带,其中有99条为多态性条带,多态位点百分率为93.40%。数据表明所分析的油橄榄种质具有丰富的遗传多样性。根据各品种的Nei’s遗传距离,运用UPGMA方法构建了所有品种的聚类图,结果 48个油橄榄品种被聚成了4个大类;84.6%的国内选育品种聚于2个类群中;多数引种品种并没有按照地理起源而是依据引种材料来源地和主要用途聚类。研究结果为油橄榄种质资源的利用以及进一步的良种选育提供参考。Inter-simple sequence repeat （ ISSR ） molecular markers were applied to analyze genetic diversity and clustering of 48 introduced and breeding olive cultivars. 106 bands were amplified by 11 selected primers, among which 99 bands were polymorphic. Percentage of polymorphic loci was 93.40%, which showed a rich genetic diversity in the collection. Based on Nei＇ s genetic distances, dendrogram of 48 cuhivars was eonstrueted through unweighted pair-group method （ UPMGA ） . 48 cuhivars were clustered into 4 main groups. 84.6% of native select and breeding cultivars were clustered into 2 groups and most of introduced cuhivars were clustered based on their directly introducing places and main usage. The results will provide references for olive resource utilization and further genetic improving.

关 键 词：油橄榄 遗传多样性 聚类分析 

分 类 号：S565.7[农业科学—作物学]