脊索瘤组织中Brachyury表达与预后的关系  

Relationship between expression of Brachyury in chordoma tissue and prognosis

作　　者：张琳琳[1,2] 张展[1,2] 段震峰[2] 

机构地区：[1]郑州大学第三附属医院检验科,郑州450052 [2]哈佛大学麻省总医院骨肉瘤分子生物学实验室,波士顿02114

出　　处：《郑州大学学报（医学版）》2013年第2期204-207,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨脊索瘤组织中Brachyury的表达及意义。方法:征集78例脊索瘤组织、7例脊索瘤癌旁正常组织和6例胚胎脊索组织用于制作组织芯片,采用免疫组化法检测Brahcyury的表达。采用回顾性分析,临床追踪57例患者,其中无病存活患者27例,带瘤存活患者7例,因脊索瘤死亡患者23例。结果:Brachyury在脊索瘤组织中的阳性表达率为75.64%(59/78),与癌旁正常组织中的4/7比较差异无统计学意义(H=22.669,P=0.263),其在骶骨发病者的阳性表达水平高于发病于活动脊柱者(H=2.292,P=0.022)。不同Brachyury表达水平的患者Kaplan-Meier生存曲线差异无统计学意义(χ2=0.432,P=0.933)。不同疾病状态患者脊索瘤组织中Brachyury的表达水平差异亦无统计学意义(H=2.605,P=0.272)。结论:Brachyury并不能作为预测脊索瘤预后的特异性分子标志物。Aim： To research the association of expression of Brachyury with the prognosis of chordoma. Methods： A total of 78 cases of chordoma tissues,7 cases of notochord controls near chordoma tissue and 6 cases of embryonic noto- chord tissue were collected to construct tissue microarray,and immunohistochemistry was used to detect the expression of Brachyury. A total of 57 chordoma patients were followed up,27 patients were alive without evidence of disease,7 patients were alive with disease,and 23 patients died of chordoma. Results： Brachyury expression were positive in 75. 64% （ 59 / 78） chordoma tissue,4 /7 notochord normal controls and 6 /6 embryonic notochord tissue. Brachyury positive expression level in chordoma tissue did not differ from that of notochord normal tissue（ H = 22. 669,P = 0. 263） . Brachyury positive ex- pression rate in mobile spine was lower than that in sacrum group（ H = 2. 292,P = 0. 022） . There were no significant differ- ences among patients with different disease status in Brachyury expression（ H = 2. 605,P = 0. 272） ,and the survival curves of patients with different Brachyury expression level had no difference（ χ 2 = 0. 432,P = 0. 933） . Conclusion： Brachyury can not be used as specific biomarker to predict the prognosis of chordoma.

关 键 词：脊索瘤 BRACHYURY 预后 

分 类 号：R738.1[医药卫生—肿瘤]