Preservation efficiency of new cryoprotectant used for Acidithiobacillus ferrooxidans in liquid nitrogen  

一种用于嗜酸性氧化亚铁硫杆菌液氮保藏的新型保护剂的保藏效果(英文)

作　　者：吴学玲[1] 胡琪[1] 侯冬梅[1] 辛小红[2] 苗博[1] 王洋洋[1] 刘学端[1] 申丽[1] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]武汉大学生命科学院中国典型培养物保藏中心,武汉430072

出　　处：《Transactions of Nonferrous Metals Society of China》2013年第3期818-823,共6页中国有色金属学报（英文版）

基　　金：Project(2005DKA21208) supported by the R&D Infrastructure and Facility Development Program from the Ministry of Science and Technology of China;Project(2010CB630901) supported by the National Basic Research Program of China

摘　　要：The efficiency of a new cryoprotectant,GP,for the preservation of Acidithiobacillus ferrooxidans（A.ferrooxidans） strain DC in liquid nitrogen was investigated.The optimal concentration of this new cryoprotectant for the maximal viable cell recovery and the highest ferrous ion oxidation activity was determined.The results show that 30%（volume fraction） GP is optimal for the cryopreservation with 84.4% of cells surviving,completely oxidizing ferrous ions within 120 h,and growing to a final density of 5.8×107 cell/mL after 6 d in the culture.Furthermore,the optimal residual GP concentration for viable cell recovery after culture of thawed cells in 9K medium for 6 d is 0.6%（volume fraction）.At this concentration,strain DC completely oxidizes ferrous ions within 108 h and grows to a final cell density of 6.8×107 mL-1.Thus,GP is a simple,effective cryoprotectant for the preservation of A.ferrooxidans strain DC in liquid nitrogen.对一种用于嗜酸性氧化亚铁硫杆菌液氮冷藏新型保护剂GP的保藏效果进行研究。依据最大细胞复苏率及最高亚铁氧化活性确定该新型保护剂的最佳使用浓度。结果表明,保护剂的最佳浓度为30%,在此浓度下细胞复苏率达到84.4%,且能在120h内完全氧化培养基中的亚铁,培养6d后菌体浓度达到5.8×107cell/mL。此外,解冻细胞在9K培养基中培养6d后,对活细胞复苏的最佳GP残留浓度为0.6%(体积分数)。在此浓度下,菌株DC完全氧化亚铁需要108h,并且最终菌体浓度为6.8×107cell/mL.因此,GP是一种简单、有效的嗜酸性氧化亚铁硫杆菌液氮保藏的冷冻保护剂。

关 键 词：Acidithiobacillus ferrooxidans CRYOPROTECTANT liquid nitrogen freezing preservation efficiency 

分 类 号：Q93[生物学—微生物学]