CRYOPROTECTANT

作品数：19被引量：21H指数：3
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相关领域：医药卫生更多>>
相关作者：侯冬梅苗博胡琪刘学端王洋洋更多>>
相关机构：中南大学武汉大学更多>>
相关期刊：《Biosafety and Health》《Chinese Journal of Chemical Engineering》《World Journal of Hepatology》《Chinese Physics B》更多>>
相关基金：国家自然科学基金中国博士后科学基金天津市自然科学基金湖北省自然科学基金更多>>
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Evaluation of microRNA expression profiles in human sperm frozen using permeable cryoprotectant-free droplet vitrification and conventional methods
《Asian Journal of Andrology》2024年第4期366-376,共11页Li-Xin Zhang Jing Mao Yan-Dong Zhou Guang-Yao Mao Run-Fa Guo Hong-Shan Ge Xia Chen 
For sperm cryopreservation,the conventional method,which requires glycerol,has been used for a long time.In addition,the permeable cryoprotectant-free vitrification method has been continuously studied.Although the di...
关键词：CRYOPRESERVATION cryoprotectant-free vitrification early embryonic development paternal epigenetic inheritance sperm miRNAs SUCROSE 
Update on techniques for cryopreservation of human spermatozoa被引量：4
《Asian Journal of Andrology》2022年第6期563-569,共7页Chuan Huang Yu-Lin Tang Jian-Ling Hu Wen-Jun Zhou Zeng-Hui Huang Xue-Feng Luo Zheng Li Wen-Bing Zhu 
supported by the National Natural Science Foundation of China(No.82001634);the China Postdoctoral Science Foundation(No.2019M661521).
In the 1960s,sperm cryopreservation was developed as a method to preserve fertility.Currently,techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction.However,although sp...
关键词：cryodamage CRYOPROTECTANT dry preservation freezing and thawing sperm cryopreservation 
Probiotics viability in frozen food products被引量：1
《Food Bioscience》2022年第6期1193-1203,共11页Nikita Tyutkov Anastasia Zhernyakova Artyom Birchenko Elmira Eminova Liudmila Nadtochii Denis Baranenko 
supported by the Russian Science Foundation(grant No.21-16-00124,https://rscf.ru/en/project/21-16-00124/).
Improving gastrointestinal flora with probiotics is a promising method of treating and preventing many diseases.However,in food industry,introduction of probiotics is limited by the shelf life of products,as well as b...
关键词：Probiotic Frozen food Probiotic viability ENCAPSULATION CRYOPROTECTANT 
Influencing factors on the preservation of lytic bacteriophage VP3被引量：2
《Biosafety and Health》2022年第5期314-320,共7页Yue Xiao Pin Huang Zhenzhou Huang Keyi Yu Yang Song Ning Guo Hang Dai Mengnan Jiang Yi Xu Duochun Wang Qiang Wei 
National Science and Technology Major Project on Important Infectious Diseases Prevention and Control(2018ZX10734404);National Science and Technology Infrastructure of China(National Pathogen Resource Center‐NPRC‐32);National Science and Technology Fundamental Resources Investigation Program of China(2021FY100904);National Nature Science Foundation of China(82161148010‐4,52076140).
Long-term and stable preservation of bacteriophages is of crucial importance.Although many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is...
关键词：Influencing factors PRESERVATION Lytic bacteriophage CRYOPROTECTANT 
Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells被引量：3
《Bioactive Materials》2021年第12期4377-4388,共12页Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 
This work was partially supported by grants from the US National Science Foundation(CBET-1831019 to XH and DGE-1840340 that is a GRFP fellowship to SS);National Institutes of Health(NIH R01EB023632 to XH).
Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contempor...
关键词：SAND Ice seeding CRYOPRESERVATION iPSC Stem cell 
Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa
《Asian Journal of Andrology》2021年第2期150-156,共7页Odai AM Alqawasmeh Mingpeng Zhao Carol PS Chan Maran BW Leung Ki C Chow Nikunj Agarwal Jennifer SM Mak Chi C Wang Chi P Pang Tin C Li Wai K Chu David YL Chan 
The Chinese University of Hong Kong Direct Grant for their partial support for this research(grant No.4054351).
Cryopreservation impairs sperm quality and functions,including motility and DNA integrity.Antioxidant additives in sperm freezing media have previously brought improvements in postthawed sperm quality.Green tea extrac...
关键词：ANTIOXIDANTS cryopreservation additives sperm cryopreservation sperm DNA fragmentation sperm motility 
Improving native human sperm freezing protection by using a modified vitrification method被引量：2
《Asian Journal of Andrology》2021年第1期91-96,共6页Dai Zhou Xing-Ming Wang Rui-Xue Li Yi-Ze Wang Yuan-Chi Chao Zhi-Zhong Liu Zeng-Hui Huang Hong-Chuan Nie Wen-Bing Zhu Yue-Qiu Tan Li-Qing Fan 
This work was supported by National Natural Science Foundation of China(No.31472054);the National Key Research and Development Program of China(2016YFC1000600).
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa in clinical practice.However,it has been shown to have a negative impact on sperm function and structure.Vitrification as a suc...
关键词：CRYOPRESERVATION CRYOPROTECTANT slow freezing SPERM VITRIFICATION 
Exploring novel cell cryoprotectants based on neutral amino acids被引量：2
《Chinese Journal of Chemical Engineering》2020年第10期2640-2649,共10页Xiaojie Sui Pengguang Chen Chiyu Wen Jing Yang Qingsi Li Lei Zhang 
the financial support from the National Natural Science Foundation of China(Nos.21621004,21961132005,21908160 and 21422605);the Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0407);the Tianjin Natural Science Foundation(18JCYBJC29500);the China Postdoctoral Science Foundation(2019M651041)。
Cryoprotectants play a key role in cell cryopreservation because they can reduce cryoinjuries to cells associated with ice formation.To meet the clinical requirements of cryopreserved cells,cryoprotectants should be b...
关键词：Biomedical engineering Cell engineering Tissue engineering CRYOPRESERVATION CRYOPROTECTANT Neutral amino acid 
A Review on the Protection Mechanism of Trehalose on Plant Tissues and Animal Cells被引量：1
《Agricultural Biotechnology》2019年第4期29-34,共6页Yiwen WANG Shujing QUAN Huan MA Dehai LIU Fuhong XIE Guocan CHEN Baitao WANG Mingli AN Fang DING 
Supported by Henan Provincial Key Project(154200510025,182102210354,182102310665,18ZP05001)
Trehalose is a non-reducing disaccharide composed of glucose molecules connected byα-glycosidic bond.This soluble substance plays an important role of protecting green algae and other lower plants from stress.It can ...
关键词：TREHALOSE ABIOTIC STRESS SIGNAL MOLECULE CRYOPROTECTANT Action mechanism 
Growth and Freeze-Drying Optimization of Bifidobacterium crudilactis
《Food and Nutrition Sciences》2016年第7期616-626,共11页Jean Tanimomo Véronique Delcenserie Bernard Taminiau Georges Daube Catherine Saint-Hubert Alain Durieux 
Bifidobacterium crudilactis FR62/b/3 belongs to a new population of bifidobacteria isolated from raw milk and raw milk cheese. The objective of this work was to study the large scale culture of the strain and its stab...
关键词：Probiotic BIFIDOBACTERIA Crudilactis CRYOPROTECTANT FREEZE-DRYING 
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