Update on techniques for cryopreservation of human spermatozoa  

作　　者：Chuan Huang Yu-Lin Tang Jian-Ling Hu Wen-Jun Zhou Zeng-Hui Huang Xue-Feng Luo Zheng Li Wen-Bing Zhu 

机构地区：[1]Institute of Reproductive and Stem Cell Engineering,Basic Medicine College,Central South University,Changsha 410000,China [2]Reproductive and Genetic Hospital of CITIC-Xiangya,Changsha 410000,China [3]Department of Andrology,Center for Men's Health,Shanghai General Hospital,Shanghai Key Lab of Reproductive Medicine,Shanghai 200025,China

出　　处：《Asian Journal of Andrology》2022年第6期563-569,共7页亚洲男性学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.82001634);the China Postdoctoral Science Foundation(No.2019M661521).

摘　　要：In the 1960s,sperm cryopreservation was developed as a method to preserve fertility.Currently,techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction.However,although sperm cryobiology has made notable achievements,the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive.Postthawing sperm quality can be affected by cryoprotectants,ice formation,storage conditions,and osmotic stress during the freezing process.This review discusses recent advances in different cryopreservation techniques,cryoprotectants,and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.

关 键 词：cryodamage CRYOPROTECTANT dry preservation freezing and thawing sperm cryopreservation 

分 类 号：R697[医药卫生—泌尿科学]