Exploring novel cell cryoprotectants based on neutral amino acids  

作　　者：Xiaojie Sui Pengguang Chen Chiyu Wen Jing Yang Qingsi Li Lei Zhang 

机构地区：[1]Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China [2]Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China [3]Qingdao Institute for Marine Technology of Tianjin University,Qingdao 266235,China

出　　处：《Chinese Journal of Chemical Engineering》2020年第10期2640-2649,共10页中国化学工程学报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China(Nos.21621004,21961132005,21908160 and 21422605);the Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0407);the Tianjin Natural Science Foundation(18JCYBJC29500);the China Postdoctoral Science Foundation(2019M651041)。

摘　　要：Cryoprotectants play a key role in cell cryopreservation because they can reduce cryoinjuries to cells associated with ice formation.To meet the clinical requirements of cryopreserved cells,cryoprotectants should be biocompatible,highly efficient and easily removable from cryopreserved cells.However,integration of these properties into one cryoprotectant still remains challenging.Herein,three biocompatible neutral amino acids,includingβ-alanine,γ-aminobutyric acid andε-aminocaproic acid,are first reported to have the potential as such ideal cryoprotectants.The results demonstrate that they can inhibit ice formation and reduce osmotic stress to provide extracellular and intracellular protection,thereby ensuring high cryopreservation efficiency for both anuclear and nucleated cells.More importantly,due to the remarkable osmotic regulation ability,the neutral amino acids can be rapidly removed from cryopreserved cells via a one-step method without causing observable damage to cells,superior to the current state-of-the-art cryoprotectants—dimethyl sulfoxide and glycerol.This work provides a new perspective to develop novel cryoprotectants,which may have dramatic impacts on solvent-free cryopreservation technology to support the cell-based applications,such as cell therapy and tissue engineering,etc.

关 键 词：Biomedical engineering Cell engineering Tissue engineering CRYOPRESERVATION CRYOPROTECTANT Neutral amino acid 

分 类 号：Q2-33[生物学—细胞生物学]