乳腺癌细胞中p73ɑ与ERɑ的相互关系  

Co-relation between ERɑ and p73ɑ in breast cancer cells

作　　者：张佳娜[1,2] 汪庆余[1] 曾磊[1] 陈青[1,2] 陈婧[3] 李庆[1] 雷浪[1] 王莉[1,2] 郝华[1] 苏晓燕[1] 冯琼[1] 刘繁荣[1] 李里香[1] 张文昌[1] 徐高四[4] 邬黎青[1,2] 

机构地区：[1]南昌大学第二附属医院病理科,江西南昌330006 [2]南昌大学第二附属医院分子医学中心,江西南昌330006 [3]南昌大学护理学院,江西南昌330006 [4]南昌大学第二附属医院肾内科,江西南昌330006

出　　处：《实验与检验医学》2013年第1期4-6,9,共4页Experimental and Laboratory Medicine

基　　金：国家自然科学基金资助项目(项目编号:30960430);江西省自然科学基金资助项目(项目编号:20114BAB205053)

摘　　要：目的探讨乳腺癌细胞中ERɑ蛋白与p73ɑ蛋白之间物理上及功能上的相互关系。方法采用蛋白印迹法检测ERɑ与p73ɑ的蛋白表达;用免疫共沉淀法检测ERɑ蛋白与p73ɑ蛋白之间的相互结合;用荧光素酶分析实验进一步检测ERɑ与p73ɑ基因的转录活性,以探讨ERɑ与p73ɑ蛋白表达改变的原因。结果 (1)经蛋白酶抑制剂MG132处理细胞后,ERɑ蛋白及p73ɑ蛋白能结合成复合体。(2)共转染ERɑ与p73ɑ时,ERɑ及p73ɑ能相互抑制彼此蛋白表达。结论乳腺癌细胞中ERɑ及p73ɑ能结合形成复合体并能相互抑制彼此蛋白的表达。Objective To investigate the physical and functional relation between ERɑ and p73ɑ in breast cancer cells.Methods Protein expressions of ERɑ and p73ɑ were examined by Western Blot.The binding assay for ERɑ and p73ɑ was tested with Co-IP and immunoblot.Transcriptional activity of ERɑ and p73ɑ was analysed by Luciferase assay.Results and Conclusion（1）ERɑ and p73ɑ can bind to each other.（2）ERɑ and p73ɑ can be combined to form complex and mutually inhibit protein expression of each other in breast cancer cells.

关 键 词：乳腺癌 ERɑ p73ɑ 相互结合 

分 类 号：R739.7[医药卫生—肿瘤]