桑椹汁对结肠癌细胞株SW620增殖和凋亡的影响  

The Influence of Mulberry Fruit Juice on Proliferation and Apoptosis of Colon Cancer Cell Line SW620

作　　者：吕欢[1] 闻燕[1] 王俊[1] 许晓风[1,2] 吴宇[1] 江明珠[1] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]南京师范大学生命科学学院,南京210046

出　　处：《蚕业科学》2013年第2期394-399,共6页ACTA SERICOLOGICA SINICA

基　　金：江苏科技大学引进人才科研启动项目(No.35211002);江苏省科技支撑计划(农业)项目(No.BE2010419)

摘　　要：为证实桑椹的抗肿瘤功能,以不同浓度桑椹汁处理结肠癌细胞株SW620,应用MTT法对比绘制生长曲线,调查SW620细胞的增殖情况,通过荧光定量PCR、SDS-PAGE检测SW620细胞凋亡相关基因p53的转录及蛋白质表达变化,分析桑椹汁对SW620细胞增殖、凋亡的影响机制。结果显示,桑椹汁对SW620细胞增殖的抑制作用随着桑椹汁浓度的升高而明显增强,以花青素质量浓度为6.267 3×10-3mg/mL的桑椹汁处理24 h后SW620细胞发生了以下变化:细胞的生长明显受到抑制,细胞脱落、变形与贴壁性下降,出现凋亡现象;细胞基因组DNA发生较明显的降解;荧光定量PCR检测细胞中p53基因的转录水平较对照组增加约6.5倍,SDS-PAGE检测p53蛋白的表达量也明显高于对照组。研究结果表明,桑椹汁对结肠癌细胞株SW620的增殖有抑制作用,其抑制机制可能是通过其活性物质诱导SW620细胞中p53基因上调表达导致细胞凋亡。To confirm anti-tumor function of mulberry fruit,colon cancer cell line SW620 was treated with different concentrations of mulberry fruit juice.The growth curve was drawn by MTT method to investigate proliferation of SW620 cells.Change of transcriptional level of apoptosis-related gene p53 in SW620 cells and expression level of its encoded protein were detected by fluorescent quantitative PCR and SDS-PAGE to analyze the influencing mechanism of mulberry fruit juice on proliferation and apoptosis of colon cancer cell line SW620.It was found that the inhibitory effect of mulberry fruit juice on the proliferation of SW620 cells was enhanced obviously with the increase of mulberry fruit juice concentration.After being treated with mulberry fruit juice containing 6.267 3×10-3 mg/mL anthocyanin for 24 h,SW620 cells had the following remarkable changes.Cell growth was obviously inhibited.Cells exfoliated and deformed.Cell adherence declined and cell apoptosis was observed.Genomic DNA of SW620 cells showed obvious degradation.The result of fluorescent quantitative PCR showed that expression level of gene p53 in the experimental group increased by 6.5 folds compared with the control group.In addition,SDS-PAGE assay indicated that the expression level of protein p53 in the experimental group was also obviously higher than that in the control group.These results indicated that mulberry fruit juice has inhibitory effect on proliferation of colon cancer cell line SW620.The inhibitory mechanism may be related to cell apoptosis caused by up-regulation of p53 gene in SW620 cells which was induced by active ingredients of mulberry fruit juice.

关 键 词：桑椹汁 结肠癌细胞株SW620 增殖 凋亡 P53基因 荧光定量PCR 

分 类 号：R735.35[医药卫生—肿瘤] R285.5[医药卫生—临床医学]