青土霉素废水出水对斑马鱼抗氧化指标的影响  

Effects of Wastewater Effluent of Penicillin and Oxytetracycline on Antioxidant Indexes of Danio rerio

作　　者：沈洪艳[1,2] 吴志刚[2] 高吉喜[3] 武晨虹[2] 王丽新[2] 

机构地区：[1]北京师范大学水科学研究院,北京100875 [2]河北科技大学环境科学与工程学院,河北石家庄050080 [3]国家环保部南京环境科学研究所,江苏南京210042

出　　处：《河南科技大学学报（自然科学版）》2013年第3期78-82,111,共5页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家环保部公益基金项目(201109004);河北省科技厅基金项目(12276708D);河北省教育厅重点基金项目(ZH2011119);河北省重点学科建设基金项目

摘　　要：采用暴露试验方法,研究了青霉素、土霉素混合废水出水不同暴露浓度对斑马鱼肌肉抗氧化指标超氧化物歧化酶(SOD)和丙二醛(MDA)的影响。研究结果表明:SOD活性指标在1/16φ(96hLC50)变化明显(P<0.05);混合废水出水在暴露后期(8～10 d)对斑马鱼肌肉MDA影响显著,8 d时对机体产生了明显的氧化损伤,暴露10 d时,机体的氧化损伤明显减轻。1/16φ(96hLC50)可能是该废水中抗生素发生作用的一个转折点,在青霉素、土霉素混合废水出水作用10 d时,可使斑马鱼肌肉SOD活性显著下降,并对斑马鱼的抗氧化能力有显著影响。斑马鱼肌肉中SOD和MDA对青霉素、土霉素混合废水出水暴露都较为敏感。An exposure experiment was made to study the effect of different concentrations of mixed wastewater effluent of penicillin and oxytetracycline on antioxidant indexes superoxide dismutase(SOD) activities and malondialdehyde(MDA) content in muscle tissue of Danio rerio.The results show that the activity of SOD changes obviously in 1 /16 φ(96hLC 50) group(P <0.05) compared with the control group in the same day.The effluent of mixed wastewater can have a significant effect on MDA content in the late exposure time(8 ～ 10 d).On the eighth day,the body produces a significant oxidative damage.On the exposure of tenth day,the oxidative damage is reduced obviously.1 /16 φ(96hLC 50) may be a turning point of the role of antibiotics in the wastewater.After 10 day of exposure the SOD activities are decreased significantly,and have a significant influence on the antioxidant capacity of Danio rerio.SOD and MDA are more sensitive to the mixed wastewater effluent of penicillin and oxytetracycline.

关 键 词：抗生素 混合废水 出水 斑马鱼 超氧化物歧化酶 丙二醛 

分 类 号：X17[环境科学与工程—环境科学]