嗜热脂肪芽孢杆菌β-半乳糖苷酶合成低聚半乳糖动力学模型  

Kinetic Model of Galacto-oligosaccharide Synthesis by β-galactosidase from Bacillus stearothermophilus

作　　者：陈少欣[1] 魏东芝[1] 胡振华[1] 王筱兰 俞俊棠[1] 

机构地区：[1]华东理工大学,生物反应器工程国家重点实验室生物化学研究所,上海200237

出　　处：《生物工程学报》2000年第6期735-739,共5页Chinese Journal of Biotechnology

摘　　要：根据反应机理和产物的化学结构 ,建立了嗜热脂肪芽孢来源的β 半乳糖苷酶催化乳糖水解和转半乳糖苷反应偶合的动力学模型 ,模型中包含了低聚三糖和低聚四糖的生成。通过优化计算 ,估算反应动力学参数 ,结果表明该模型能较好地与实验结果相吻合。In the course of lactose hydrolysis,the β galactosidase produced variable amounts of galacto oligosaccharide(GOS)due to the transgalactosydic reaction.According to enzymatic reaction mechanism by β galactosidase and structures of GOS,a model was developed to describe the process including GOS(trisaccharide and tetrasaccharide)formation by Bacillus stearothermophilus β galactosidase.The Simple optimization method was employed to determine the kinetic parameters of rate expression.The kinetic model showed a good agreement between experimental and simulated results.

关 键 词：Β-半乳糖苷酶 低聚半乳糖 动力学模型 

分 类 号：Q814.9[生物学—生物工程]