Effects of ivabradine on the levels of HIF-1α and VEGF in serum of rabbit with acute myocardial infarction  

作　　者：刘松 王宝魁 纪阳 徐少杰 黄玉晓 孙燕 

机构地区：[1]Department of Cardiology,Affiliated Hospital of Qingdao University Medical College [2]Qingdao HaiCi Medical Group [3]Department of Endocrinology,Affiliated Hospital of Guiyang Medical College

出　　处：《South China Journal of Cardiology》2013年第1期22-28,共7页岭南心血管病杂志（英文版）

摘　　要：The study aimed to investigate the effects of ivabradine on the levels of hypoxia-inducible factor-lalpha （HIF-1α） and VEGF in serum of rabbit with acute myocardial infarction （AMI）. Methods AMI model was established by ligating the left anterior descending branch of the coronary artery in New Zealand white rabbits. Twenty five rabbits were randomly divided into 4 groups： sham-operated （S）, myocardial-infarction （M） with bisoprolol treatment （M ＋ B） and ivabradine-treated （I ＋ M）. The medical treatment began immediately after infarction and continued for 3 weeks. Serum of each rabbit was obtained at the following time points （24 h before the operation, 24 h, 3 d, 1 week, 2 weeks and 3 weeks after the operation）. ELISA was used to measure the levels of HIF-1α and VEGF of each sample. ECG and heart rates （before and after treatment） were analyzed. Results Baseline heart rate showed no significant differences between the 3 infarcted groups （M, M ＋ B, M ＋ I）. Three weeks later the heart rates were significantly lower in group M ＋ B and group M ＋ I than in group M. However, there was no statistic difference between the two drug-treated groups （P = 0.848）. The levels of HIF-1α and VEGF in groups M, M ＋ B and M ＋ I） increased significantly compared with group S （P 〈 0.01）. The productions of HIF-1α and VEGF were lower in group M ＋ B and group M ＋ I compared with group M （P 〈 0.01）. There was no statistical difference between the group M ＋ B and group M ＋ I （P 〉 0.05）, and the correlative analysis revealed that the production of HIF-1α was positively correlated with that of VEGF （r = 0.732, P 〈 0.01）. Conclusion Ivabradine can reduce heart rate and meanwhile decrease the serum levels of HIF-1α and VEGF after AMI.The study aimed to investigate the effects of ivabradine on the levels of hypoxia-inducible factor-lalpha （HIF-1α） and VEGF in serum of rabbit with acute myocardial infarction （AMI）. Methods AMI model was established by ligating the left anterior descending branch of the coronary artery in New Zealand white rabbits. Twenty five rabbits were randomly divided into 4 groups： sham-operated （S）, myocardial-infarction （M） with bisoprolol treatment （M ＋ B） and ivabradine-treated （I ＋ M）. The medical treatment began immediately after infarction and continued for 3 weeks. Serum of each rabbit was obtained at the following time points （24 h before the operation, 24 h, 3 d, 1 week, 2 weeks and 3 weeks after the operation）. ELISA was used to measure the levels of HIF-1α and VEGF of each sample. ECG and heart rates （before and after treatment） were analyzed. Results Baseline heart rate showed no significant differences between the 3 infarcted groups （M, M ＋ B, M ＋ I）. Three weeks later the heart rates were significantly lower in group M ＋ B and group M ＋ I than in group M. However, there was no statistic difference between the two drug-treated groups （P = 0.848）. The levels of HIF-1α and VEGF in groups M, M ＋ B and M ＋ I） increased significantly compared with group S （P 〈 0.01）. The productions of HIF-1α and VEGF were lower in group M ＋ B and group M ＋ I compared with group M （P 〈 0.01）. There was no statistical difference between the group M ＋ B and group M ＋ I （P 〉 0.05）, and the correlative analysis revealed that the production of HIF-1α was positively correlated with that of VEGF （r = 0.732, P 〈 0.01）. Conclusion Ivabradine can reduce heart rate and meanwhile decrease the serum levels of HIF-1α and VEGF after AMI.

关 键 词：myocardial infarction IVABRADINE BISOPROLOL hypoxia inducible factor-1α vascularendothelial growth factors 1 

分 类 号：R542.22[医药卫生—心血管疾病]