新疆罗布泊周边胀果甘草内生芽孢杆菌BLG-542产碱性纤维素酶最佳条件的研究  

Endogenous Alkaline Fiber Bacillus of BLG-542 Production In Glycyrrhiza InflataCellulose Enzyme Optimum Conditions

作　　者：迪丽拜尔.托乎提 帕提古丽[1] 王小静[1] 刘会强[1] 黄瑞虎[1] 肉孜.阿布都热依木 

机构地区：[1]新疆师范大学极端环境微生物多样性实验室,新疆乌鲁木齐830054 [2]新疆尉犁县中学,新疆尉犁841500

出　　处：《新疆师范大学学报（自然科学版）》2013年第1期1-6,共6页Journal of Xinjiang Normal University(Natural Sciences Edition)

基　　金：国家自然科学基金项目(No.31260002)资助;新疆师范大学微生物学重点学科资助

摘　　要：文章以罗布泊胀果甘草中分离出的78个菌株中初步筛选出7株产碱性纤维素酶的菌株为研究对象,进行产碱性纤维素酶最佳优化条件的研究。碱性的胀果甘草内生芽孢杆菌菌株接种于CMC-Na为碳源,复合蛋白胨为氮源的培养基中,分析研究菌株产碱性纤维素酶的最佳条件优化。通过从78株本实验室于2006年保藏的罗布泊胀果甘草内生菌中筛选出能产生胞外碱性纤维素酶的7株菌,其中酶活力最高的菌株为BLG-542。本研究主要以酶活力最高的菌株BLG-542为出发菌株进行研究。经过对产酶条件优化,获得了胀果甘草内生菌产碱性纤维素酶最佳配方培养基:D-木糖10g/L,复合蛋白胨10g/L,可溶性淀粉10g/L,酵母粉5g/L,K2HPO41g/L,MgSO40.2g/L,NaCl 20g/L,Na2CO310g/L;实验产酶最佳接种量为6%,最佳培养温度为37℃,最佳培养时间为48h,产酶最佳PH为9.0;菌种生长最佳PH为7.6,经过优化产酶条件纤维素酶活力从优化前的3.8U/mL提高到6.26U/mL,是优化之前的1.8倍;该研究表明特殊生态环境的植物内生菌与次生代谢产物、活性物质、酶类有密切的关系,环境除对内生菌酶的产生极其活性影响外,还影响着内生菌的次生代谢。该地区植物内生菌均有潜在的应用价值。Preliminary screening in the Lop Nur Glycyrrhiza inflata isolated 20 strains of seven strains of the production of alkaline cellulase for the study, the best optimal conditions for the production of alka- line cellulase. Endogenous alkaline Glycyrrhiza inflata Bacillus strains inoculated in CMC--Na as a carbon source, the composite peptone nitrogen medium, analyzing the best optimization of conditions for strains producing alkaline cellulase. In this study, seven beads can produce extracellular alkaline cellulase isolated from Glycyrrhiza inflata 20 preserved in this laboratory Lop Nur, the highest enzyme activity of strains of BLG-542. In this study, the highest enzyme activity of strains of BLG-542 as the starting strain. After optimization of conditions for enzyme production, has been the best medium formula： D--xy- lose10 g/L, the composite peptone 10g/L, soluble starch 10g/L yeast extract 5g/L, KzHPO4 1g/L,Mg- SO4 0.2g/L, NaCI 20g/L, and Na2CO3 10g/L; optimization of experimental enzyme production optimum inoculum size 6%, the optimal incubation temperature of 37℃, 48h culture time, best PH 9.0; optimized conditions for cellulase activity from 3.8U/mL to 6.26U/mL, 1.8 times than before optimization.

关 键 词：碱性纤维素酶 胀果甘草内生菌 酶活力 产酶条件优化 

分 类 号：Q949.2[生物学—植物学]