响应面法优化垂盆草总黄酮超声提取工艺  

Optimization of Ultrasound-assisted Extraction of Total Flavonoids from Sedum Sarmentosum Bunge by Response Surface Methodology

作　　者：张本洁[1] 孙冬杰[1] 陈雨秋[1] 郑亚[1] 于小凤[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013

出　　处：《广东化工》2013年第7期12-13,11,共3页Guangdong Chemical Industry

基　　金：江苏大学大学生科研立项(11A149)

摘　　要：目的:优化垂盆草总黄酮的超声提取工艺。方法:以总黄酮得率为指标,采用响应面设计方法对影响总黄酮得率的乙醇浓度、料液比及超声温度进行优化。结果:最佳工艺条件为:采用60%乙醇,料液比26:1,在60℃下超声提取20min,在此最佳条件下,总黄酮得率为21.7mg?g-1,实验结果与模型预测值相符。结论:利用超声方法提取垂盆草总黄酮工艺稳定可靠。Objective：To optimize the ultrasound-assisted extraction technology of total flavonoids from Sedum sarmentosum Bunge.Method：The factors such as ethanol concentration,liquid-material ratio and extraction temperature were optimized by response surface methodology.Result：The highest extraction rate of total flavonoids by ultrasound-assisted extraction could arrive at 21.7 mg？g-1 using 60 % ethanol concentration,liquid-material ratio of 26：1,extraction temperature 60 ℃,and extraction time 20 min,which was agreed with model predictions.Conclusion：The extraction process by ultrasound-assisted was stable and reliable.

关 键 词：垂盆草 总黄酮 超声提取 响应面设计 

分 类 号：R284.2[医药卫生—中药学]