SPATA12基因在多种肿瘤中的组织芯片表达谱分析  

Analysis of the SPATA12 Gene Expression Pattern in Multiple Tumors Using Tissue Micro-array

作　　者：李丹[1] 章运生[1] 刘志文[1] 林奕婷[1] 荣卓献[1] 刘选明[1] 

机构地区：[1]湖南大学生物学院生命科学系,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2013年第4期75-79,共5页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(3087276381270735);中央高校基本科研业务费专项基金项目(2010-208)

摘　　要：为了检测生精相关基因SPATA12在多种肿瘤组织中的表达及其与临床病理特征的关系,采用数字虚拟Northern方法分析SPATA12在41种正常组织及其相应肿瘤组织中的表达丰度;利用组织芯片技术结合原位杂交的方法,对96例多器官肿瘤组织芯片和208例肺肿瘤组织芯片样本中SPATA12基因的表达情况进行检测.虚拟Northern结果显示,SPATA12主要表达于正常睾丸组织以及少部分肺癌组织中.组织芯片原位杂交结果显示,SPATA12在多种肿瘤组织中表达频率不一,主要在皮肤恶性黑色素瘤、前列腺癌及前列腺慢性炎症组织、胃癌、结肠癌、甲状腺乳头状癌等肿瘤组织中高表达;在肺肿瘤组织中,SPATA12主要表达于非小细胞肺癌,其中在腺癌、鳞癌、类癌和大细胞癌组织的阳性表达率分别为16.13%,29.17%,30%和100%.SPATA12的阳性表达率与肺癌不同病理类型相关(P<0.01),而与年龄、性别以及临床分级无关(P>0.05).SPATA12基因具有肿瘤-睾丸抗原的组织表达谱特点,其阳性表达与肺肿瘤组织的不同病理类型存在明显的相关性,对肺肿瘤的分子分型有一定的参考价值.To investigate the expression pattern of human spermatogenesis associated gene 12（SPATA12）and its correlation with clinicopathological features in multiple tumors,Virtual Northern analysis based on EST in 41 human normal tissues and corresponding tumor tissues was firstly used to evaluate digital mRNA profiling of SPATA12.Then in situ hybridization was performed to detect the expression of SPATA12 in 96 cases of multiple-organ tumor tissues and 208 cases of lung tumor tissues by using tissue micro-array.Virtual Northern results show that SPATA12 is predominantly expressed in normal testis and slightly expressed in lung cancers.In situ hybridization results demonstrate that SPATA12 gene has different expression frequency in multiple tumor tissues.SPATA12 is highly expressed in the cutaneous malignant melanoma,prostatic carcinoma and chronic inflammation of prostate,gastric cancer and its cancer-adjacent tissues,prostatic carcinoma and its cancer-adjacent tissues,papillary thyroid carcinoma and pancreatic ductal adenocarcinoma.In lung tumors,the positive rates of SPATA12 expression in lung adenocarcinoma,squamous cell carcinoma,carcinoid and large cell lung cancer tissues were 16.13%,29.17%,30%,100%,respectively.There was a significant difference between SPATA12 expression and different pathological casificated lung cancers（P0.01）,but no significant difference among age,gender and clinical stages（P0.05）.The expression pattern of SPATA12 gene is consistent with the expression feature of cancer/testis antigens family.And the expression of SPATA12 in lung tumor patients indicate that SPATA12 is closely related to pathological classification.Detection of SPATA12 might be helpful to evaluate molecular classification for lung tumors.

关 键 词：SPATA12基因 基因表达 肿瘤-睾丸抗原 

分 类 号：R730.21[医药卫生—肿瘤]