CCl_4诱导的急性肝损伤小鼠肝组织HSP27表达的变化  

The changes of heat shock protein-27 expression in mice with CCl_4 induced acute liver injury

作　　者：乔伟强[1] 李三强[1] 孙永健[1] 贾志强[2] 贾岩岩[1] 丁腾腾[1] 周洁[1] 

机构地区：[1]河南科技大学医学院肝脏损伤与修复分子医学重点实验室,河南省洛阳市471003 [2]辽宁省锦州市辽宁医学院

出　　处：《实用肝脏病杂志》2013年第2期150-151,共2页Journal of Practical Hepatology

基　　金：河南科技大学大学生研究训练计划项目(编号:2012189)

摘　　要：目的研究热休克蛋白(HSP)27在CCl4诱导的急性肝损伤小鼠肝组织中的动态变化。方法将100只健康雄性小鼠随机分为模型组90只和对照组10只。应用CCl4腹腔注射诱导小鼠急性肝损伤,在3h、6h、12h、24h、30h、36h、42h、48h和54h及正常对照小鼠分别眼球取血或取肝脏制备10%肝匀浆,采用Western blotting法检测肝组织HSP27表达的变化。结果小鼠注射CCl4后,血清AST和ALT活性逐渐升高,24h达到高峰,54h逐渐恢复到正常水平;肝组织HSP27在注射CCl4后3h表达量略有增高,在24h表达量达到高峰,此后逐渐恢复到正常水平。结论在CCl4诱导的急性肝损伤小鼠肝组织HSP27表达出现显著变化,可能在肝损伤的修复和再生过程中起到重要的作用。Objective To study the dynamic changes of heat shock protein（HSP）-27 in mice with CCL-in- duced acute liver injury. Methods One hundred mice were randomly divided into 10 groups. The acute liver in- jury of mice were induced by CCh abdominal injection. Eye blood and liver tissues were respectively collected at 3h,6h,12h,24h,30h,36h,42h,48h and 54h after CCl4 injection. Western blotting was used to detect the expres- sion of HSP27 during acute liver injury in mice. Results AST and ALT activities were gradually increased and got to the peak levels at 24h,then they gradually recovered to the normal level at 54h after CCl4 injection;The hepatic expression of HSP27 was slightly increased at 3h and got to the peak levels at 24h （P〈O.O5）,theu the HSP27 expression gradually returned to normal levels. Conclusion The expression of HSP27 is significantly changed during acute liver injury in mice induced by CCl4,which may play an important role in the process of regeneration of liver after liver injury.

关 键 词：急性肝损伤 CCL4 热休克蛋白-27 

分 类 号：R575[医药卫生—消化系统]