大蒜素对耐碳青霉烯类抗菌药物鲍曼不动杆菌体外抑菌作用的研究  

Inhibitory effect of Allicin on the carbapenem-resistant Acinetobacter baumannii in vitro

作　　者：于亮[1] 王梅[1] 姜梅杰[1] 李玉臣[1] 刘爱华[1] 

机构地区：[1]山东省泰安市中心医院呼吸重症科,泰安市271000

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第1期39-42,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的探讨大蒜素对耐碳青霉烯鲍曼不动杆菌的杀菌作用,及其与碳青霉烯类抗菌药物(亚胺培南和美罗培南)联合使用的抑菌疗效。方法采用改良三维实验对24株多耐药鲍曼不动杆菌产生的碳青霉烯酶进行鉴定。采用MH肉汤法,确定大蒜素、美罗培南、亚胺培南,大蒜素+美罗培南和大蒜素+亚胺培南的最低抑菌浓度(MIC)值,计算两药联用的部分抑菌浓度(FIC)指数。采用微孔板生物检测法检测大蒜素、大蒜素与美罗培南、亚胺培南两药联用的抑菌率。MH肉汤法检测还原型谷胱甘肽对大蒜素抑菌作用的影响;检测与不同浓度(32、64和128μg/ml)大蒜素作用后,细菌体内谷胱甘肽、抗超氧阴离子自由基和总巯基的浓度。结果大蒜素对24株耐碳青霉烯抗菌药物鲍曼不动杆菌的MIC值均为512μg/ml,美罗培南为128μg/ml,亚胺培南为64μg/ml,两种药物联用:大蒜素+美罗培南、大蒜素+亚胺培南FIC值均为0.25,大蒜素与碳青霉烯类抗菌药物联用较碳青霉烯类抗菌药物单独使用的抑菌率显著升高。0.3mg/ml还原型谷胱甘肽+不同浓度大蒜素组较单用不同浓度大蒜素组的抑菌率显著下降。不同浓度(32、64和128g/ml)大蒜素作用后,细菌谷胱甘肽、抗超氧阴离子自由基和总巯基分别为(4.5±0.7)mgGSH/gport、(115.2±4.2)U/L、(0.11±0.04)mmol/gprot;(5.5±0.6)mgGSH/gport、(134.6±6.3)U/L、(0.18±0.06)mmol/gprot;(6.8±0.8)mgGSH/gport、(147.9±8.4)U/L、(0.24±0.02)mmol/gprot,较未应用大蒜素组差异具有统计学意义,P均<0.01。不同浓度大蒜素组之间比较,细菌谷胱甘肽、抗超氧阴离子自由基和总巯基,差异具有统计学意义,P均<0.01。结论大蒜素对耐碳青霉烯类抗菌药物的鲍曼不动杆菌抑制作用与其引起细菌体内氧化失衡有关;大蒜素与碳青霉烯类抗菌药物(亚胺培南和美罗培南)的联合使用对该细菌具有协同杀菌作用。Objective To investigate the inhibitory effect of Alliein alone and with the carbapenem antibiotics （imipenem and meropenem ） against the carbapenem-resistant Acinetobacter baumannii in vitro. Methods Improved three-dimensional experiments were applied to identify the Carbapenemases in more than 24 multiple resistant Acinetobacter baumannii. The minimal inhibitory concentration （ MIC ） values of the alliein, meropenem, imipenem were deteeted by MH broth method, respectively. The fractional inhibitory concentration （FIC） of Allicin with the carbapenem antibiotics were calculated, and the bio-detection microplate assay were applied to detect the inhibitory rates of the allicin, meropenem, imipenem and the allicin combined with two carbapenem drugs. The role of glutathione on the impact of antimicrobial effect of allicin were detected by MH broth, while the changes of glutathione, anti-superoxide anion radical and total thiol of the Acinetobacter baumannii were detected after different concentrations of allicin incubation. Results All 24 carbapenem resistant Acinetobacter baumannii produced Carbapenemases by improved three-dimensional experiments. Antibiotic MIC value of Allicin, meropenem and imipenem on 24 earbapenem-resistant Acinetobacter baumannii were 512 μg/ml, 128 μg/ml and 64 μg/ml, respectively. FIC of the allicin with two carbapenem drugs were both 0. 25, compared with carbapenem alone, the inhibition rates of Allicin and carbapenem in combination was significantly increased. The inhibition rate of allcin against bacterials was significantly decreased in 0.3 mg/ml GSH ＋ different concentrations of allicin groups compared with allicin group. The glutathione, anti-superoxide anion radical and total thiol were （4.5 ± 0.7） mgGSH/gport, （115.2 ± 4.2） U/L, （0.11 ± 0.04） mmol/gprot in 128 μg/ml allicin groups ; （5.5 ± 0.6） mgGSH/gport, （ 134.6 ± 6.3） U/L, （0.18 ± 0.06） mmol/gprot in 64 μg/ml allicin groups; （6.8± 0.8） mgGSH/gport, （147.9 ± 8.4） U/L, （0

关 键 词：大蒜素 碳青霉烯抗菌药物 鲍曼不动杆菌 还原型谷胱甘肽 

分 类 号：R285[医药卫生—中药学]