用放射免疫测定法分析水中莠去津残留量的研究  

Analysis Atrazine Residues in Water Using Radioimmunoassay

作　　者：徐德武[1] 朱永清 杨根海[3] 楚慧民 阎美玉 

机构地区：[1]莱阳农学院动物科学系,山东莱阳265200 [2]菏泽农校,山东菏泽274000 [3]中国农业大学,北京100094 [4]济宁市农科所,山东济宁272100 [5]莱阳市环保局,山东莱阳265200

出　　处：《莱阳农学院学报》2000年第3期225-228,共4页Journal of Laiyang Agricultural College

摘　　要：以莠去津均三氮苯类结构特征 ,合成连有活性羧基的莠去津半抗原 ,通过活性酯法制备出具有生物活性的人工抗原 ,以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强 ,与扑灭津的交叉反应性为 93 %,与西玛津的交叉反应性为 8%。以3H标记莠去津建立的莠去津放射免疫测定法对水中莠去津添加回收率的测定表明 ,自来水的添加回收率为 88.5%～ 1 0 7.5%,运河水的添加回收率为 87.4 %～1 1 0 .9%。The determination of atrazine residue in water with radioimmunoassay method was studied. The High bioactive antigen was synthesized and the high titer anti-atrazine antibody was prepared. The antiserum was specific for atrazine,the cross-reactivity of the antiserum to propazine and simazine was 93% and 8%,respectively. The RIA for atrazine labeled by 3H was established and showed that the rate of recovery by adding atrazine in drink water and yunhe water were 88.5%-107.5% and 87.4%-110.9%respectively.

关 键 词：莠去津 放射免疫测定法 水质监测 残留量 

分 类 号：X132[环境科学与工程—环境科学] X832