刺五加注射液诱导宫颈癌HeLa细胞凋亡机理的研究  

Mechanism study on apoptosis induced by acanthopanax senticosus injection on cervical cancer HeLa cells

作　　者：王倩[1] 赵刚[1,2] 

机构地区：[1]湖北中医药大学基础医学院,武汉430065 [2]中药复方与中药资源省部共建教育部重点实验室

出　　处：《公共卫生与预防医学》2013年第2期13-17,共5页Journal of Public Health and Preventive Medicine

基　　金：湖北省教育厅科学技术研究重点项目(D20101808);湖北省教育厅科学技术研究计划优秀中青年人才项目(Q20121606);湖北中医药大学校级科研课题中青年项目

摘　　要：目的探索刺五加注射液诱导宫颈癌细胞HeLa发生凋亡的效应和分子机理。方法以不同浓度的刺五加注射液作用于体外培养的宫颈癌HeLa细胞24 h,应用AO/EB双染的荧光显微镜观察法和Annexin V-FITC染色的流式细胞技术,检测不同浓度刺五加注射液诱导HeLa细胞凋亡的形态改变和凋亡率;应用免疫细胞化学方法,检测HeLa细胞凋亡蛋白酶caspase-3和caspase-8的表达水平。结果刺五加可诱导HeLa细胞发生凋亡,并随着药物浓度升高细胞凋亡率增加;荧光显微镜观察发现,在刺五加的作用下,HeLa细胞数量明显减少,呈现特征性的凋亡形态;免疫细胞化学检测显示,HeLa细胞的caspase-3和caspase-8的表达水平随刺五加浓度的增加而升高,呈现剂量依赖性。结论刺五加注射液诱导HeLa细胞凋亡的机理可能涉及了caspase依赖性的细胞凋亡信号途径。Objective To investigate apoptosis induced by Acanthopanax senticosus injection on cervical cancer HeLa cells and its mechanism in vitro. Methods The HeLa Cells were treated with different dosage of acanthopanax senticosus injection for 24 hours. The apoptotic rate of HeLa cells induced by acanthopanax senticosus injection was detected by flow cytometry based on Annexin V-FITC/PI staining. Morphological changes of apoptosis based on AO/EB staining were observed with fluorescent micro- scope. The expression level of caspase-3 and caspase-8 were detected by immunocytochemistry method. Results Flow cytome- try analysis showed that acanthopanax senticosus injection could induce apoptosis of HeLa Cells,the apoptotic rate increased with the increase of concentration. The AO/EB staining showed that the total number of HeLa cells decreased with the increase of acanthopanax senticosus injection concentration,and part of the ceils showed apoptotic morphological characteristics obviously. The immuno-histochemistry staining indicated that the expression of caspase-3 and caspase-8 proteins increased in acanthopanax senticosus injection and showed a concentration-dependent manner. Conclusions The HeLa Cells apeptosis induced by acanthopanax senticosus injection could be achieved through caspase-dependent pathway.

关 键 词：刺五加 HELA细胞 CASPASE 凋亡机理 

分 类 号：R713.4[医药卫生—妇产科学]