干扰赖氨酰氧化酶基因对乳癌 MDA-MB-231细胞生物学的影响  

Influence of interfering lysyl oxidase gene on biological features of MDA-MB-231 cells

作　　者：李德全[1] 李景涛[1] 刘剑仑[1] 杨华伟[1] 

机构地区：[1]广西医科大学附属肿瘤医院乳腺外科,南宁530021

出　　处：《中华实验外科杂志》2013年第4期664-666,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81160321）;广西科学研究与技术开发计划课题（桂科攻0816004-14）

摘　　要：目的观察抑制赖氨酰氧化酶（LOX）基因表达后对MDA-MB-231细胞的生物学特性的影响。方法将细胞分为实验组、空白对照组和阴性对照组，采用病毒转染法，应用噻唑蓝（MTT）法、细胞集落形成实验、流式细胞仪和细胞侵袭迁移实验检测结果。结果24、48、72、96h3 组细胞抑制率分别是（12. 73 ±3. 30）% .（33.93 ± 1. 99）% ,（52. 63 ± 1.04）% ,（63. 80 ±0. 70）%;0、0、0、0;（7. 60 ±6. 27）%、（ 10. 30 ± 1. 49）%、（9.10 ±2. 69）%、（6. 03 ± 1. 21）%，差异有统计学意义（P 〈0.05）。细胞周期时相差异无统计学意义（P〉0.05）。细胞侵袭实验结果分别为47±2、100±1、88±2,差异有统计学意义（P〈0.05）;细胞迁移实验结果分别为63 ±2,118 ±2,96 ±2，差异有统计学意义（P〈0. 05） 0结论干扰LOX基因的表达可抑制MDA-MB-231细胞的生物学特性。Objective To observe the influence of RNAi against lysyl oxidase （LOX） gene on bi-ological features of MDA-MB-231 cells. Methods The MDA-MB-231 cells were transfected with lentiviralinterference vector （ LOX-RNAi-LV）,and divided into three groups ： the experimental group, the blankgroup and the negative group. The growth and proliferation of cells were determined by using methyl thiazoltetrazolium （ MTT） assay, cell colony formation and flow cytometry. The migration and invasion were ob-served by using transwell assays. Results The cell growth rate in the experimental group, the blank groupand the negative group at 24, 48, 72 and 96 h was （12. 73 ± 3. 30） % , （ 33. 93 ± 1. 99） %,（52. 63 ±1.04）% , （63. 80 ±0.70）%; 0,0,0,0; （7. 60 ±6. 27）%,（10. 30 ±1.49）%, （9. 10 ±2. 69）%,（6. 03 ± 1. 21 ） % , respectively （ P 〈 0. 05 ） . Cells invading between three groups was 47 ± 2; 100 ± 1;88 ±2 （p 〈0. 05） . Cell migration was 63 ±2; 118 ±2; 96 ±2 （p 〈0. 05）. The difference of the cell cy-cle was not statistically significant （P 〉 0. 05）. Conclusion After the LOX gene expression restrained,thecreature feature of MDA-MB-231 were effectively suppressed.

关 键 词：乳癌 赖氨酰氧化酶基因 RNA干扰 生物学特性 

分 类 号：R737[医药卫生—肿瘤]