CC趋化因子受体7、血管内皮生长因子-C表达对非小细胞肺癌淋巴结转移潜能的影响  

Effect of CC chemokine receptor 7 and vascular endothelial growth factor-C expression on lymph node metastatic potentiality of non-small cell lung cancer

作　　者：陈钢[1] 刘相燕[1] 王洲 刘凡英[1] 

机构地区：[1]山东大学附属省立医院胸外科,济南250021

出　　处：《中华实验外科杂志》2013年第4期735-737,共3页Chinese Journal of Experimental Surgery

基　　金：山东省自然科学基金资助项目（Y2007CL06）

摘　　要：目的观察CC趋化因子受体7（CCR7）及血管内皮生长因子-C（VEGF-C）的表达对非小细胞肺癌（NSCLC）淋巴结转移潜能的影响。方法临床收集行肺癌完全切除加系统淋巴结清扫的55例NSCLC患者的肺癌组织、淋巴结组织及正常肺组织标本。逆转录-聚合酶链反应（RT-PCR）检测CCR7及VEGF-C mRNA的表达;免疫组织化学检测CCR7及VEGF-C蛋白的表达。应用X2检验比较CCR7及VEGF-C表达差异，并应用Logistic回归分析判定肺癌淋巴结转移的独立相关因素。结果肺癌组织中有39例（70.9%）CCR7 mRNA及32例（58.2%）VEGF-CmRNA表达阳性，有38例（69. 1%）CCR7蛋白及29例（52. 7% ） VEGF-C蛋白表达阳性，且均与淋巴结转移密切相关（P〈0.05）。33例有转移的淋巴结中CCR7、VEGF-C mRNA的阳性表达率分别为84.8%、66.1%，分别明显高于无转移的淋巴结（27. 3% ,18. 2%，P〈Q. 05）。Logistic多因素回归分析结果显示：肺癌组织中CCR7 mRNA的阳性表达[比值比（洲）=10. 275，P 〈0. 01 ]及VEGF-C mRNA的阳性表达（OR=5.550，p〈0.05）是NSCLC患者淋巴结转移的独立危险因素。结论CCR7及VEGF-C在NSCLC患者的肺癌组织及淋巴结转移灶中表达均明显增高，并与肺癌淋巴结转移密切相关；GCR7及VEGF-C的表达可能促进了 NSCLC淋巴结转移。Objective To explore the effects of CC chemokine receptor 7 （CCR7） and vascularendothelial growth factor-C （VEGF-C） expression on lymph node metastatic potentiality of non-small celllung cancer （NSCLC）. Methods The samples of cancer tissues,lymph nodes and normal lung tissuesfrom 55 patients with NSCLC who underwent complete resection were examined by using reverse tran-scriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry to detect CCR7 and VEGF-C ex-pression. The difference in the CCR7 and VEGF-C expression was compared by^2 test. Logistic regressionanalysis was performed to determine the independent risk factors of lymph node metastasis of NSCLC. Re-sults In lung cancer tissues,CCR7 mRNA was expressed in 39 cases （70. 9% ） and VEGF-C mRNA in32 cases （58. 2% ）,and CCR7 protein was expressed in 38 cases （69. 1% ） and VEGF-C protein in 29cases （52. 7% ） , which was positively correlated to lymph node metastasis （ P 〈 0. 05 ） . The expressionrate of CCR7 and VEGF-C in metastatic lymph nodes （27. 3% and 18. 2% respectively） was higher thanin non-metastatic lymph nodes （ P 〈 0. 05 ） . Logistic regression analysis revealed that CCR7 mRNA expres-sion [odds ratio （ OR） = 10. 275,P 〈0. 01 ] and VEGF-C mRNA expression in tumors （ OR = 5. 550,P 〈0. 05） were independent risk factors of lymph node metastasis of NSCLC. Conclusion CCR7 and VEGF-Cwere highly expressed in lung cancer and the metastatic lymph nodes, with significant correlation withlymph node metastasis of NSCLC. The expression of CCR7 and VEGF-C promotes lymphatic metastasis oflung cancer and might be a clinical predictor of lymph node metastasis of patients with NSCLC.

关 键 词：CC趋化因子受体7 血管内皮生长因子-C 非小细胞肺癌 淋巴结转移 

分 类 号：R734.2[医药卫生—肿瘤]