RP-HPLC法测定西洋参浸膏和西洋参茶中人参皂苷Rg1与Re的含量  

DETERMINATION OF GINSENOSIDES RG1 AND RE IN EXTRACT OF PANAX QUIQUEFOLIUM L.AND PANAX QUINQUEFOLIUM L.TEA BY RP-HPLC

作　　者：马鹏[1] 张强[1] 王秀兰[2] 肖娟[2] 

机构地区：[1]华西医科大学药学院,成都610041 [2]华西医科大学制药厂,成都610041

出　　处：《天然产物研究与开发》2000年第1期54-57,共4页Natural Product Research and Development

摘　　要：报道了西洋参浸膏和西洋参茶中人参皂苷 Rg1与 Re的反相高效液相色谱( RP- HPL C)测定法 ,用十八烷基硅烷键合相柱 ,UV 2 0 3 nm检测 ,流动相为乙腈 -0 .0 5%磷酸溶液 ( 18∶ 82 )。比较了样品的纯化方法。WT5BZ]An RP HPLC method for the determination of Ginsenosides Rg1 and Re in extract of Panax quinquefolium L.and Panax quinquefolium L.tea was reported.Using Inertsil ODS 2(150×4 6 mm I.D.) column with acetonitril 0 05% phosphoric acid solution(18∶82) as mobile phase,detection UV 203 nm.Sample purification was also discussed.The established method was proved to be rapid,sensitive and have a good reproducibility.

关 键 词：反相高效液相色谱 西洋参浸膏 西洋参茶 

分 类 号：TS272.55[农业科学—茶叶生产加工]