辣椒素引起脑干内心血管活动相关核团中c-fos的表达  

Increased expression of c-fos in the brainstem nuclei involved in cardiova scular regulation by capsaicin

作　　者：薛保健[1] 张小雪[1] 石葛明[1] 何瑞荣[1] 

机构地区：[1]河北医科大学基础医学研究所生理室,1解剖教研室石家庄050017

出　　处：《生理学报》2000年第2期159-162,共4页Acta Physiologica Sinica

摘　　要：在 16只切断两侧缓冲神经的大鼠 ,观察颈总动脉注射辣椒素对脑干内心血管活动相关核团c fos原癌基因表达的影响。在溶剂对照组大鼠脑干 ,仅见少数Fos蛋白样免疫反应 (FLI)神经元。与对照组相比 ,颈总动脉注射辣椒素 ( 10 μmol,0 1ml)时 ,脑干内巨细胞旁外侧核 (PGL)、蓝斑 (LC)、最后区 (AP)和孤束核 (NTS)等部位的FLI神经元显著增加 ,而中脑中央灰质 (PAG)和中缝核群 (RN)的FLI神经元则无明显改变。应用辣椒素受体阻断剂钌红 (RR ;2 0 0mmol,0 1ml) ,可明显抑制辣椒素的上述效应。以上结果提示 ,辣椒素可通过激活辣椒素受体 (VR) ,引起脑干内多个心血管活动相关核团的神经元兴奋 。Effects of intracarotid injected capsaicin on the expression of Fos proto oncogene in the brainstem nuclei involved in cardiovascular regulation were examined in 16 anesthetized rats with sinoaortic denervation. Only a few Fos like protein immunoreactivity (FLI) neurons were found in the vehicle control rats. Following intra^carotid injection of capsaicin (10 μmol, 0 1 ml), the FLI neurons were markedly increased in nucleus paragigantocellularis lateralis (PGL), locus coeruleus (LC), area postrema (AP) and nucleus tractus solitarius (NTS), as compared with the results of vehicle control rats, while the FLI neurons of raphe nuclei (RN) and periaqueductal gray (PAG) were not affected. The excitatory response to intracarotid injection of capsaicin was significantly inhibited by pretreatment with the vanilloid receptor (capsaicin receptor) antagonist ruthenium red (200 mmol, 0 1 ml). The results indicate that capsaicin may activate the neurons of the brainstem nuclei involved in cardiovascular regulation through vanilloid receptors, but does not exert any effects on PAG and RN.

关 键 词：辣椒素 钌红 Fos样蛋白 脑干 

分 类 号：Q463[生物学—生理学] R331.3[医药卫生—人体生理学]