利用RAPD标记研究荔枝品种的亲缘关系  

IDENTIFYING LITCHI CULTIVARS AND EVALUATING THEIR GENETIC RELATIONSHIPS BY RAPD MARKERS

作　　者：丁晓东[1] 吕柳新[2] 陈晓静[2] 关雄[3] 

机构地区：[1]东北农业大学园艺系,黑龙江哈尔滨150030 [2]福建农业大学园艺系,福建福州350002 [3]福建农业大学生物技术中心,福建福州350002

出　　处：《热带亚热带植物学报》2000年第1期49-54,共6页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金;福建省自然科学基金

摘　　要：采用改进的ＣＴＡＢ方法提取３４个荔枝品种的基因组ＤＮＡ为模板，从５０个１０碱基的随机引物中获得了３７个多态性ＲＡＰＤ标记，聚类分析结果表明这３４个荔枝品种可以分成４组，第一组有“状元红”、“元红”、“桂林”、“宋家香”、“台湾荔”、“下番枝”、“东刘一号”、“妃子笑”、“白糖罂”、“香丸”、“绿沙”；第二组有“无栗子”、“水东”、“乌叶”、“蔡家肉丸”、“青壳糯米糍”、“糯米糍”；第三组有“桂味”、“光明”、“白荔枝”、“陈紫”、“兰竹”、“及第”、“踏死牛”；第四组有“回头降”、“葡萄本”、“淮枝”、“南海荔”、“大造”、“早红”、“火烧荔”、“乌叶舅”、“密丁香”、“绿荷包”．In this paper, we used modified CTAB method to extract genomic DNA from litchi for amplifying RAPD markers. We got 37 polymorphic RAPDs out of 259 total RAPDs from 34 litchi cultivars with 50 random primers. The results showed that these 34 cultivars could be divided into 4 groups. Group I included 'Zhuangyuanhong', 'Yuanhong', 'Guilin', 'Songjiaxiang', 'Taiwanli', 'Xiafanzhi', 'Dongliu No. l', 'Feizixiao', 'Baitangying', 'Xiangwan', 'Lusha'. Group II comprised 'Wulizi', 'Shuidong', 'Wuye', 'Caijiawanzi', 'Qingkenuomici', 'Nuomici'. Group III included 'Guiwei' ,'Guangming','Bailizhi','Chenzi' ,'Lanzhu','Jidi','Tasiniu'. Group IV consisted of 'Huitoujiang', 'Putaoben', 'Huaizhi','Nanhaili','Dazao', 'Zaohong', 'Huoshaoli', 'Wuyejiu', 'Midingxiang', 'Luhebao'. The results of cluster suggested that the polymorphism of litchi germplasm was narrower than expected.

关 键 词：荔枝 RAPD 聚类分析 品种 亲缘关系 

分 类 号：Q943[生物学—植物学]