基于微机械电子系统的电化学电极阵列的研制  

Development of Electrochemical Array Based on Micro Electro-Mechanical Systems

作　　者：赵朝辉[1] 王力[1] 甘先云 朱昌安[3] 刘成桂[1] 郑军[1] 谢国明[1] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016 [2]重庆精科仪表制造有限责任公司,重庆400700 [3]中国电子科技集团第26研究所,重庆400060

出　　处：《分析化学》2013年第4期621-626,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.81171415);重庆市教委科学基金(No.120320)资助项目

摘　　要：为了克服传统电化学电极反应液需要量较大、预处理繁琐和耗时的不足,本研究通过腐蚀、键合、光刻、氧化、扩散和溅射等工艺在75 mm×25 mm光学玻片上制备了基于微机械电子系统的电化学电极阵列。该电极阵列含有16个电极单元,每个单元之间的差异性≤3.7%;电极表面Rct=28Ω,预处理时间1 min;反应液量1～50μL。用此电极阵列构建的生物传感器实现了对大肠杆菌特异性靶分子序列的检测,检测下限达到1×10-8mol/L。结果表明,此电化学电极阵列能满足多通道微量检测的需求,在其表面可方便地进行生物功能化修饰,应用范围广,实用性强。In order to improve the complex,time consuming pretreatment and large substrate liquid needing of the traditional electrode,we proposed the electrochemical array.By means of technologies of corrosion,linkage,photolithography,oxidation diffusion and sputtering,the array was fabricated on an optical glass.The variations were less than 3.7% among 16 Au electrode units.It took only 1 min of the pretreatment and the reaction liquid volume was 1-50 μL.The Rct was 28 Ω of the electrochemical array.Functionalized electrochemical array was used to detect the specific molecule sequence of Escherichia Coli with a low detection limit of 1×108 mol/L.It was proved that electrochemical array has a superior practicability,its surface could be easily functionalized and could satisfy the need of multichannel trace detection.

关 键 词：电化学电极阵列 微机械电子系统 循环伏安法 计时电流法 DNA分子探针 

分 类 号：O657.1[理学—分析化学]