海洋胶红酵母Y2产类胡萝卜素发酵促进剂的研究  

Studies on the promoters of carotenoid fermentation by Rhodotorula mucilaginosa

作　　者：杨铿[1] 陈永青[1] 梁晓华[1] 杨莺莺[1] 佘妙虹[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《南方水产科学》2013年第2期45-49,共5页South China Fisheries Science

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2010ZD02);现代农业(虾)产业技术体系建设专项资金(CARS-47);公益性行业(农业)科研专项(201103034);广东省鱼病防治专项(2130108);农业科技成果转化资金项目(2012GB23260551)

摘　　要：研究了几种添加物对海洋胶红酵母(Rhodotorula mucilaginosa)Y2发酵产类胡萝卜素的促进作用。在摇瓶条件下,发酵培养基中分别添加不同量的番茄汁、花生油、大豆油、玉米浆、谷氨酸钠和生物素,培养72 h后取样分别测定类胡萝卜素产量及生物量。结果显示,当分别添加2 mL.L-1番茄汁、1.5 mL.L-1花生油和3 mL.L-1玉米浆时,类胡萝卜素产量比对照组提高14.2%、30.3%和21.3%;谷氨酸钠和生物素会抑制海洋胶红酵母Y2类胡萝卜素的合成。因此,番茄汁、花生油和玉米浆是较为经济适用的海洋胶红酵母Y2产类胡萝卜素发酵促进剂,具有广阔的开发应用潜力。We studied the effects of several additives on the yield of carotenoid fermented by Rhodotorula mucilaginosa. In the shake flask condition, different concentrations of tomato juice, peanut oil, soybean oil, corn steep liquor, sodium glutamate and biotin were added to the fermentation medium respectively, and the carotened yields as well as R. mucilaginosa biomass were measured after 72 h. The results show that when 2 mL＇L-1 tomato juice, 1.5 mL-L-lpeanut oil, and 3 mL＇L-1 corn steep liquor are added, the yields of carotenoids are increased by 14. 2%, 30. 3% and 21.3% respectively. However, sodium glutamate and biotin may inhibit the synthe- sis of carotenoid by R. mucilaginosa. Therefore, tomato juice, peanut oil and corn steep liquor are good promoter for carotenoid fermen- tation by R. mucilaginosa, which may have great potential for applications in the future.

关 键 词：海洋胶红酵母Y2 类胡萝卜素 生物量 发酵促进剂 

分 类 号：Q935[生物学—微生物学]