不同性质大肠粘膜细胞凋亡与增殖的研究  

A study on apoptosis and proliferation in different colon mucosa

作　　者：胡文清[1] 徐德龙[1] 王克诚[1] 肖文华[1] 魏梅新[1] 

机构地区：[1]河北医科大学第三医院外科,石家庄050051

出　　处：《中国肿瘤临床与康复》2000年第4期27-28,共2页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　研究不同性质大肠粘膜细胞中细胞凋亡与增殖状态及其意义。方法　取正常大肠粘膜 10例 ,大肠腺瘤 2 0例 ,大肠癌远旁、近旁和癌粘膜各 3 0例。应用TUNEL、PCNA免疫组化染色 ,在连续切片上同步检测细胞凋亡和增殖状态。结果　TUNEL染色阳性物质定位于细胞核 ,固缩成团、聚成环状或形成凋亡小体。PCNA染色阳性物质也主要定位于细胞核 ,呈弥漫或颗粒状着色。正常大肠粘膜、大肠腺瘤和大肠癌细胞凋亡率 ,各组间比较均有统计学意义 (P <0 .0 1) ;PCNA阳性细胞数 ,各组间比较均有极显著性差异 (P <0 .0 1)。轻、中、重度不典型增生大肠腺瘤细胞凋亡率比较 ,轻度与中度、重度不典型增生比较有显著性差异 (P <0 .0 1) ;PCNA阳性细胞数 ,各组间比较均有极显著性差异(P <0 .0 1)。大肠癌远旁、近旁和癌组织中细胞凋亡率 ,各组间比较均有极显著性差异 (P <0 .0 1) ;PCNA阳性细胞数 ,各组间比较也均有极显著性差异 (P <0 .0 1)。细胞凋亡与增殖与大肠癌的分化程度、分期和淋巴结转移有关 ;与组织学类型无关。 3 0例大肠癌细胞凋亡率和PCNA的表达呈极显著性正相关关系 (P <0 .0 0 1)。 2 0例大肠腺瘤细胞凋亡率和PCNA的表达呈正相关关系 (P <0 .0 1)。结论　细胞凋亡与增殖是反映细胞周转率的指标 ;检测细胞凋?? Objective To study on the significance of apoptosis and proiferation in genesis and development of human colon carcinoma (HCC).Methods TUNEL and PCNA immunohistochemical staining was used to test apoptosis or proliferation of different mucosal cells in 30 cases of HCC,20 cases of colon adenoma and 10 cases of normal control.Results Positive staining of both kind was located in cell nuclear.Apoptosis rate was significantly higher in HCC than in colon adenoma (P<0.01) and in nomal mucosa (P<0.01).PCNA positive percentage was significantly different among each group (P<0.01).Apoptosis and positive percentage of PCNA had significant correlation (P<0.001).Conclusions Apoptosis rate and PCNA expression level in HCC are closely associated with the differentiation,clinical stage,metastasis and prognosis of the tumor,and thus probably become objective markers in predicting prognosis of HCC. 〔

关 键 词：细胞凋亡 增殖细胞核抗原 大肠腺瘤 大肠癌 

分 类 号：R730.2[医药卫生—肿瘤]