老年直肠癌患者术后外周血Treg T的临床研究  

Effect of rectal cancer resection on regulatory CD4^+ CD25^+ T lymphocytes in peripheral blood of older patients with rectal carcinoma

作　　者：范惠珍[1] 夏红梅[2] 尹卫华[3] 陈金平[3] 潘永东[2] 盛建文[1] 

机构地区：[1]宜春学院临床医学院(宜春市人民医院)消化科,江西宜春330006 [2]宜春学院临床医学院(宜春市人民医院)普外科,江西宜春330006 [3]宜春学院临床医学院(宜春市人民医院)肿瘤科,江西宜春330006

出　　处：《中国现代医学杂志》2013年第5期51-54,共4页China Journal of Modern Medicine

基　　金：江西省自然科学基金(No:20111BBG70014-9)

摘　　要：目的探讨直肠癌切除术对老年直肠癌患者外周血Treg T细胞的影响及临床意义。方法宜春市人民医院自2006年9月～2011年9月有60例施行手术的老年直肠癌患者,采用流式细胞术检测患者手术前及术后1个月外周血中Treg占单核细胞(PBM)的比例,同样的方法检测正常直肠组织和直肠癌组织中Treg占单核细胞的比例;采用ELISA检测正常直肠组织和直肠癌组织中转化生长因子-β(TGF-β)、白介素-10(IL-10)的水平。结果直肠癌患者外周血和直肠癌组织中Treg占单核细胞比例明显高于正常对照组(12.32±0.32)%VS(8.85±0.11)%,(6.30±0.21)%VS(3.43±0.11)%(P<0.05);Ⅲ、Ⅳ期直肠癌组患者外周血和直肠癌组织中Treg占单核细胞比例高于Ⅰ、Ⅱ期组患者(16.43±0.56)%VS(10.34±0.28)%,(8.78±0.43)%VS(5.6±0.13)%(P<0.05)。此外,直肠癌组织中IL-10和TGF-β水平高于正常直肠组织;Ⅲ、Ⅳ期直肠癌组患者肿瘤组织中IL-10和TGF-β高于Ⅰ、Ⅱ期直肠癌组织,差异具有统计学意义(P<0.05),说明直肠癌局部Treg细胞数及其分泌IL-10和TGF-β细胞因子与肿瘤TNM分期呈正相关。结论 Treg可作为老年直肠癌患者免疫功能状态的一个客观标识;其数量与肿瘤TNM呈正相关,是决定肿瘤预后的一个独立危险因素。【Objective】 To explore the effect of rectal cancer resection on regulatory CD4 ＋ CD25 ＋ T lymphocytes（Treg） in peripheral blood and its clinic significance in for patients with rectal carcinoma.【Methods】 Collected 60 older patients with rectal carcinoma in the People＇s Hospital of Yichun city in Jiangxi province from Steptemper 2006 to Steptemper 2011,which were included in our study.It was used the flow cytometer to analyze the percent of Treg in peripheral blood mononudear cell before and affer surgery,and the ratio of Treg to PBM in tumor tissue and normal rectal tissue.In addition,enzyme-linked immunosorbent assay（ELISA） was used to detect the level of TGF-β,IL-10 in tumor tissue and normal rectal tissue.【Results】 The percentage of Treg in PBM and tumor tissue of rectal carcinoma patients were both higher than those in the normal groups（12.32 ± 0.32） % VS（8.85 ± 0.11） %,（6.30 ± 0.21） % VS（3.43 ± 0.11） %,（P 0.05）. Moreover,the percentage of Treg in phase Ⅲ～Ⅳ patients was higher than that in phase Ⅰ～Ⅱ（P 0.05）. Similarity,TGF-β and IL-10 in tumor tissue were significantly higher than those in normal rectal tissue,additionally,TGF-β and IL-10 in tumor tissue was higher in phase Ⅲ～Ⅳ patients than those in phase Ⅰ～Ⅱ（P 0.05）.It was positive correlated with TNM classification that the level of Treg in patients with rectal cancer significantly increased.【Conclusion】 The level of Treg was considered to objective identification and an independent prognostic factor in older patients with rectal carcinoma,which was positive correlated with TNM classification.

关 键 词：老年直肠癌患者 直肠癌 调节性T细胞 转化生长因子 白细胞介素10 

分 类 号：R735.37[医药卫生—肿瘤]