C反应蛋白-757T／C基因多态性与缺血性脑血管病患者颈动脉粥样硬化的相关性  

The association between C-reactive protein -757T/C gene polymorphism and carotid artery artherosclerosis in patients with ischemic cerebrovascular disease

作　　者：袁海成[1] 许波[1] 陈晶[1] 王岭[1] 李俊芳[2] 汪东[1] 李从真[1] 

机构地区：[1]青岛大学医学院第二附属医院神经科,266042 [2]青岛大学医学院第二附属医院超声科,266042

出　　处：《中华神经科杂志》2013年第4期224-228,共5页Chinese Journal of Neurology

摘　　要：目的探讨C反应蛋白-757T／C基因多态性与缺血性脑血管病患者颈动脉粥样硬化的相关性。方法采用病例对照研究的方法，对276例缺血性脑血管病患者及125名对照者（两组成员性别、年龄均匹配）应用聚合酶链反应．限制性片段长度多态性对C反应蛋白-757T／C基因位点进行分析，采用免疫散射比浊法测定血清C反应蛋白水平，应用彩色多普勒超声测定缺血性脑血管病患者颈总动脉及颈内动脉内膜-中膜厚度（IMT），分析c反应蛋白-757T／C基因型、等位基因型与颈动脉粥样硬化的关系。结果缺血性脑血管病组患者C反应蛋白-757T／C基因型TT、TC和cc的频率分布[207／276（75．0％）、65／276（23．6％）和4／276（1．4％）]与对照组[75／125（60．0％）、46／125（36．8％）和4／125（3．2％）]比较，差异具有统计学意义（X2=9．531，P=0．008）；缺血性脑血管病患者c反应蛋白-757T等位基因频率[479／552（86．8％）]高于对照组[196／250（78．4％）]，而C等位基因频率低于对照组[73／552（13．2％）与54／250（21．6％）]，差异具有统计学意义（x2=9．056，P=0．004）；-757TC／CC基因型患者血清C反应蛋白水平较TT基因型患者明显增高[（4．2±1.6）mg／L与（2．7±1．9）mg／L；t=5．900，P〈0．01]；Logistic回归分析表明年龄、糖尿病、血清C反应蛋白水平及低密度脂蛋白胆固醇是颈动脉IMT增厚的独立的危险因素，并且c反应蛋白-757多态位点C等位基因与颈动脉粥样硬化具有相关性（OR=1．379，95％C10．852～0．864，P=0．023）。结论C反应蛋白-757T／C基因多态性与缺血性脑血管病有关，C反应蛋白-757C等位基因能够影响血清c反应蛋白水平；Logistic回归分析表明C反应蛋白-757T／C基因多态性与颈动脉粥样硬化相关。Objective To explore the relationship of a polymorphism c. 757T 〉 C in C-reactive protein gene （CRP） and carotid artery atheroselerosis in patients with ischemie cerebrovascular disease （ICVD）. Methods A case-control study investigated 276 ICVD patients and 125 controls matched for age and sex. Genotypes of- 757T/C in CRP were analyzed in patients with ICVD using polymerase chain reaction-restriction fragment length polymorphism. The level of serum C-reactive protein was detected by using immune scatter tnrbidimetry and the intima-media thickness of common carotid artery and internal carotid artery were measured by color Doppler ultrasonography. The relationship between the polymorphism -757T/C in CRP and carotid atherosclerosis was assessed. Results The genotypes of the polymorphism - 757T/C in CRP in the ICVD group （ 207/276 （ 75.0% ）, 65/276 （ 23.6% ）, 4/276 （ 1.4% ） ） was significantly different from the control group （75/125 （ 60. 0% ）, 46/125 （ 36. 8% ）, 4/125 （ 3.2% ） ; x2 = 9.531, P = 0. 008 ）, the frequence of T allele in patients with ICVD （479/552 （86. 8% ） ） was increased significantly compared to the control group （ 196/250 （78.4%） ） , the frequence of C allele in the ICVD group （73/552（ 13.2% ） ） was decreased significantly in contrast to the control group （54/250 （21.6%） ; x2 = 9. 056,P = 0. 004）. Among the patients with ICVD, the level of serum C-reactive protein in patients with- 757TC/CC genotype was elevated significantly than that with -757TC/CC （（4. 2 ± 1.6） mg/L vs （2.7 ± 1.9） mg/L ; t = 5.900, P 〈 0. O1 ）. Logistic regression analysis demonstrated that the - 757C allele in CRP was related to the carotid artery atherosclerosis （ OR = 1. 379,95% CI O. 852-0. 864, P = 0. 023 ）. Conclusions The polymorphism -757T/C in CRP maybe related to the risk of developing [CVD. The frequency of the - 757C allele can increase the level of serum C-reactive protein. And this polymorphism may be associated with carot

关 键 词：脑缺血 C反应蛋白质 多态现象 遗传 颈动脉疾病 

分 类 号：R743.3[医药卫生—神经病学与精神病学]