梓醇对淀粉样蛋白前体／早老素1双转基因小鼠老年斑和学习记忆的影响  

Effect of catalpol on senile plaques and spatial learning and memory ability in amyloid-β protein precursor/presenilin 1 double transgenic mice

作　　者：宋冲[1] 楚亚楠[1] 贺桂琼[1] 刘刚[1] 王凌晞[1] 周泽芬[1] 姚秋会[1] 

机构地区：[1]重庆医科大学神经生物学重庆市重点实验室,400016

出　　处：《中华神经科杂志》2013年第4期265-268,共4页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目（30970986）;教育部“新世纪优秀人才”支持计划资助项目（NCET-11-1084）;重庆市首届优秀人才资助计划项目[渝教人（2009）2号];留学人员科技活动项目择优资助项目[渝人社办（2011）235号]

摘　　要：目的观察梓醇对淀粉样蛋白前体／早老素1（APP／PS1）双转基因小鼠老年斑和学习记忆能力的影响。方法将3个月龄的APP／PSI双转基因小鼠按照随机数字表法分为梓醇治疗组和生理盐水对照组，每组10只，并以10只同月龄的相同遗传背景的C57小鼠作为正常对照组。用梓醇（每天5mg/kg）和等量生理盐水腹腔注射阿尔茨海默病（AD）模型小鼠3周，应用免疫组织化学检测各组小鼠老年斑数量的变化；应用Morris水迷宫检测小鼠空间学习记忆能力的变化。结果免疫组织化学结果显示：与生理盐水对照组（6．0±0．6）比较，梓醇治疗组小鼠老年斑数量（个）明显减少（2．3±0．7，t=3．500，P=0．025）。行为学结果显示：（1）在可视平台实验中，3组小鼠找到平台的平均潜伏期和搜索的平均路径差异无统计学意义。（2）隐蔽平台下，梓醇治疗组小鼠找到平台的时间及搜索的路径较生理盐水对照组小鼠明显缩短；与正常对照组比较，差异无统计学意义。（3）在探索实验中，60s内梓醇治疗组（6．4±0．5）小鼠穿越平台次数（次）明显高于生理盐水对照组（2．9±0．4，t=5．592，P=0．001），而与正常对照组（6．8±0．6）比较差异无统计学意义（t=0．418，P=0．682）。结论梓醇治疗能显著减少AD模型小鼠脑内老年斑数量，改善小鼠的空间学习记忆能力。Objective To investigate whether catalpol affects senile plaque formation and spatial learning and memory ability in the amyloid-β protein precursor/presenilin 1 （ APP/PS1 ） double transgenic mice. Methods Three month-old APP/PSI double transgenic mice were randomly divided into catalpol- treated and saline-treated groups （n = 10）, with C57 mice of the same age and genetic background as normal control group （ n = 10）. The catalpol （ in a dose of 5 mg kg-1 d -1 ） and the same amount of saline were peritoneally injected into Alzheimer＇ s disease （AD） model mice for 3 weeks. Immunohistochemical staining was performed to examine senile plaques in the brain of AD model mice, and Morris water maze was used to assess the spatial learning and memory abilities of the mice. Results Compared with the saline-treated AD model mice （6. 0 ± 0. 6）, the number of senile plaques of catalpol treated AD mice significantly decreased （2. 3 ± 0. 7 ; t = 3. 500, P = 0. 025 ） ; Mice in each groups had similar latency and path length to reach platform in visible platform test; In hidden platform test, catalpol-treated mice had a significant lesser latency and path length compared with saline-treated mice, furthermore, catalpol-treated mice had much more platform-crossing times （6.4 ± 0. 8） than saline-treated mice （ 2. 9 ± 0. 4 ; t = 5. 592, P = 0. 001 ）. Conclusion Catalpol can significantly decrease the senile plaque formation and improve the spatial learning and memory abilities of APP/PS1 double transgenie mice.

关 键 词：梓醇 淀粉样Β蛋白前体 早老素1 小鼠 转基因 衰老斑 学习 记忆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]