r-H2AX在不同食管癌株系中的剂量时间效应特点  

Expression of r-H2AX in Time-effect and Dose-response between Different Esophageal Carcinoma Cell Lines

作　　者：史鸿云[1,2] 祝淑钗[1] 卢付河[1] 王玉祥[1] 苑兰惠[2] 何丽[1] 

机构地区：[1]河北医科大学附属第四医院放疗科,石家庄050017 [2]河北大学附属医院放疗科

出　　处：《肿瘤防治研究》2013年第4期321-326,共6页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30870743)

摘　　要：目的通过检测不同食管癌株系的r-H2AX时间剂量效应关系,了解r-H2AX的动力学特点。方法将食管癌ECA109和TE13两种细胞株分别给予1、2、4和8Gy的6MV-X线照射,并分别于0.5、1、2、4、8、12、24 h收集细胞提取蛋白,检测r-H2AX时间及剂量效应特点。结果 (1)ECA109细胞和TE13细胞随着放疗剂量的增加,r-H2AX表达量第一次最高点出现的时间逐渐提前。(2)ECA109细胞接受低剂量照射后,r-H2AX在24 h内能够恢复到放疗前的水平,且剂量越低恢复越快;TE13细胞接受1、2、4和8Gy照射后24 h内r-H2AX均不能恢复到放疗前水平。(3)在0.5、1、2 h这3个时间点,随着放疗剂量的增加,r-H2AX的表达量并不都是逐渐增加。结论 TE13细胞较ECA109细胞敏感,照射后r-H2AX更难恢复到正常水平。Objective To investigate the time-effect and dose-effect of r-H2AX in different esophageal carcinoma lines after irradiation.Methods The dose-response（1,2,4,8Gy） and time-effect of r-H2AX in different esophageal carcinoma lines after irradiation were studied and compared by Western blot.Two cell lines of esophageal carcinoma（ECA109 and TE13） were radiated by 6MV-X ray of（1,2,4 and 8Gy） The cells and extracted proteins at 0.5,1,2,4,8,12 and 24 h were collected and to study the dose-response and time-effect relationship of r-H2AX.Results（1） The first peak of r-H2AX expression gradually appeared in advance with the increasing radiation dose of ECA109 cells and TE13 cells.（2） r-H2AX within 24 hours recovered to the level before radiotherapy after low-dose irradiation in ECA109,The level recovered faster if the dose of radiation is lower;r-H2AX can not be recovered to the level before radiotherapy after irradiation of 1,2,4 and 8Gy within 24 h in TE13.（3） At 0.5,1 and 2 h,the expression of r-H2AX in ECA109 and TE13 cells didn＇t increase with the increase in radiation dose.Conclusion The level of r-H2AX is more difficult to return to normal levels in TE13 cell line than in ECA109 cell line after irradiation which shows that TE13 is more sensitive to irradiation.

关 键 词：食管癌 r-H2AX 时间效应 剂量效应 

分 类 号：R735.1[医药卫生—肿瘤]