5-氮杂-2-脱氧胞苷对人胰腺癌细胞株PANC-1侵袭和迁移能力的影响  

Effect of 5-Aza-CdR on Invasion and Migration of Pancreatic Carcinoma Cell Line PANC-1

作　　者：王蒙 汤志刚[1,2] 黄强 任维华[1,2] 

机构地区：[1]安徽医科大学附属省立医院普外科 [2]安徽省立医院普外科,合肥230001

出　　处：《肿瘤防治研究》2013年第4期332-335,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(81071734)

摘　　要：目的研究甲基化抑制物5-氮杂-2-脱氧胞苷(5-Aza-CdR)干预对胰腺癌细胞株PANC-1侵袭和迁移能力的影响,初步探讨其发挥作用的机制。方法用不同浓度的5-Aza-CdR处理PANC-1细胞株,采用划痕迁移试验和Transwell小室法观察干预前后细胞迁移和侵袭能力的改变,免疫荧光染色法检测干预前后细胞MMP-2的表达。结果经5-Aza-CdR干预后,与对照组相比,PANC-1细胞的迁移和侵袭能力明显减弱(P<0.05),细胞内MMP-2的表达降低(P<0.05)。结论 5-Aza-CdR可以明显抑制胰腺癌细胞株PANC-1的侵袭转移能力,其机制可能是通过抑制MMP-2的表达而降低肿瘤细胞的侵袭和转移。Objective To investigate the effects of methylation inhibitor 5-Aza-2-deoxycyt（5-Aza-CdR） on the invasion and migration of pancreatic carcinoma cell line PANC-1,and to explore its possible mechanisms.Methods PANC-1 cell line was treated by 5-Aza-CdR of different concentrations.Wound scratch assay and transwell chamber were used to detect the ability of the invation and migration of PANC-1 cells.The expression of MMP-2 was detected by immunofluorescent staining.Results After being treated by 5-Aza-CdR,compared with the control group,the ability of migration and invasion was reduced obviously（P0.05）.Immunocytochemical analysis indicated that the expression of MMP-2 was down-regulated after being treated by 5-Aza-CdR（P0.05）.Conclusion 5-Aza-CdR can inhibit the invasive ability of PANC-1 cells,and its mechanism might be reducing the invasion and migration of the cells by inhibiting the expression of MMP-2.

关 键 词：胰腺癌 5-氮杂-2-脱氧胞苷 MMP-2 

分 类 号：R735.9[医药卫生—肿瘤]