人重组S100A8对牙周膜细胞增殖和迁移活性的影响  

Effect of recombinant human S100A8 on the proliferation and migration of periodontal ligament cells

作　　者：郑云飞[1] 彭磊 张欣[3] 侯建霞[1] 孟焕新[1] 

机构地区：[1]北京大学口腔医学院.口腔医院牙周科,北京100081 [2]北京市友谊医院口腔科,北京100050 [3]北京协和医院口腔科,北京100730

出　　处：《北京大学学报（医学版）》2013年第2期269-273,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30271411;30471882;30973319);"十一五"国家科技支撑计划项目(2007BAZ18B02)资助~~

摘　　要：目的:观察人钙结合蛋白轻亚基S100A8对牙周膜细胞增殖和迁移活性的影响,探讨S100A8在牙周炎发生及发展中的作用。方法:体外培养人牙周膜原代细胞,给予人重组S100A8处理,MTT检测细胞增殖活性的改变,Annexin-Ⅴ/PI染色后流式细胞检测技术观察细胞的凋亡状况,Transwell小室及划痕实验检测细胞迁移能力的改变。结果:10-7～10-5mol/L人重组S100A8抑制牙周膜细胞增殖,而10-9～10-7mol/L S100A8促进牙周膜细胞迁移,其中10-5mol/L S100A8作用牙周膜细胞48 h后可以显著抑制其增殖并诱导其凋亡,而10-9mol/L S100A8却显著促进牙周膜细胞的迁移。结论:高浓度S100A8抑制牙周膜细胞增殖且促进其凋亡,而牙周治疗后低浓度的S100A8可以促进牙周膜细胞迁移,有助于疾病后期组织的修复。Objective：To investigate the effect of S100A8 on the proliferation and migration of perio-dontal ligament cells （PDLCs）, and to learn the role of S100A8 in the development of periodontitis. Methods： PDLCs were treated with S100A8 in vitro before MTT and flow cytometry assays were per-formed. Transwell assay and wound assay were conducted to test the migratory activity of the PDLCs as well. Results： In the study, 10^-7-10^-5mol/L recombined human S100A8 suppressed the proliferation of the PDLCs, while their proliferation was significantly inhibited with 10^-5 mol/L S100A8 treatment for 48 h. And 10^-9 - 10^-7mol/L S100A8 enhanced the migratory activity of the PDLCs while the effect of 10^-9mol/L S100A8 was statistically significant. Conclusion： Increased level of S100A8 in periodontitis could lead to the inhibition of cell proliferation and apoptosis of PDLCs, but S100A8 could promote the migration of PDLCs when its concentration decreased after treatment.

关 键 词：钙粒蛋白A 钙结合蛋白质类 细胞增殖 牙周膜 牙周炎 

分 类 号：R781.42[医药卫生—口腔医学]