立体定向建立裸鼠人脑多形性胶质细胞瘤模型及生长特性研究  

THE GROWTH FEATURE OF OF BRAIN MULTIFORME GLIOBLASTOMA MODEL IN NUDE MICE WITH STEREOTAXIS ESTABLISHMENT

作　　者：田复明[1] 蒋宁[2] 窦长武[1] 王宏伟[1] 赵力群[1] 陈波[1] 王晓娟[3] 王凤[1] 

机构地区：[1]内蒙古医科大学附属医院神经外科,内蒙古呼和浩特010050 [2]内蒙古医科大学第三附属医院 [3]广东佛山科学技术学院医学院微免教研室

出　　处：《内蒙古医科大学学报》2013年第1期6-11,共6页Journal of Inner Mongolia Medical University

基　　金：内蒙古自治区卫生厅医疗卫生科研计划项目(2005074);内蒙古医科大学青年科研项目(2009)

摘　　要：目的:体外原代培养人脑多形性胶质母细胞瘤细胞,应用不同细胞数量接种裸鼠脑内建立颅内胶质瘤实验动物模型,观察其生长特性。方法:采用酶消化法原代培养4例人脑多形性胶质细胞瘤,分别取生长状态良好的胶质瘤细胞悬液20μL,按1.0×105个/10μL,1.0×106个/10μL,1.0×107个/10μL立体定向接种于裸鼠脑右侧尾状核区。在整体、组织和细胞水平观察并记录肿瘤生长情况,于成瘤后第5wk处死荷瘤鼠检测是否存在肿瘤远处转移,同时对肿瘤组织行HE和免疫组织化学染色检测胶质瘤的病理学特征。结果:细胞悬液接种可成功获得多形性胶质细胞瘤模型,成功率较高,但潜伏期延长,各种接种量的实验组成瘤率均为100%,未见颅外转移病灶,在组织病理学上接近人脑胶质瘤。结论:多形性胶质细胞瘤细胞接种裸鼠建立脑胶质瘤动物模型,成瘤率高,颅内生长稳定,肿瘤组织病理学及形态学特性与人脑胶质瘤相似,可作为临床胶质瘤基础研究的理想模型。Objective：To culture the primary human glioblastoma cells in vitro and to establish a brain glioma model in nude mice with different cell number in order to study the growth feature of glioma. Methods：The glioma ceils from digestion culture 1. 0×10^6/10μL, method. 20μL of Well 4 glioma patients were cultured primarily by the enzymatic cultured glioma cells suspension containing 1.0×10^5/10μL, 1. 0×10^7/10μL/10μL was stereotaxically inoculated into the right caudate nucleus of the rat brain. And then observed and recorded the growth of tumors in overall, tissue and cell Horizontal. Executed the nude mice at the fifth week after tumorn established, and resected their five main organs and the brain tissues. Performed HE stain and immuno-histochemistry examination to detect pathological characteristics of glioma. Results： Muhiforme glioblastoma subcutaneous model closeto SU human glioma ceess rale and gliobl rate, histopathology was successfully established by cell suspension inoculation,with high long latent period,but without other organs metastasis. Conclusion：The rat multiforme n tumor model was successfully established with highly intracranial tumor formation intrat：ranial growth stable histopathological and morphological and no extracranial growth extension. This model resembles features of human glioma,which can be used as a perfect model tostudy glioma.

关 键 词：多形性胶质母细胞瘤 动物模型 裸鼠 

分 类 号：R739.41[医药卫生—肿瘤]