脂肪酸合成酶在乳腺浸润性导管癌中的表达及其与HER2的相关性  

Expression of fatty acid synthase and its association with HER2 in invasive ductal carcinoma ofbreast

作　　者：杨明[1] 许三鹏[2] 敖启林[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院病理科,武汉430022 [2]华中科技大学同济医学院附属同济医院病理研究所

出　　处：《中华病理学杂志》2013年第4期257-261,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金（30770942）

摘　　要：目的探讨脂肪酸合成酶（FAS）在乳腺腺病、导管上皮不典型增生、原位癌及浸润性导管癌中的表达变化，观察乳腺浸润性导管癌中FAS的表达与HER2基因扩增的相关性。方法采用免疫组织化学EnVision二步法对100例各级别乳腺病变和10例癌旁正常乳腺组织进行FAS表达检测；利用荧光原位杂交（FISH）检测其中60例乳腺浸润性导管癌的HER2基因扩增情况。结果（1）100例乳腺疾病中包括腺病10例、导管上皮不典型增生10例、导管原位癌20例（高级别8例、中级别9例、低级别3例）、浸润性导管癌60例（1级5例、2级40例、3级15例）。（2）FAS在10例正常乳腺组织中呈阴性表达；10例乳腺腺病中，1例为＋＋＋，2例为＋＋，4例为＋，3例为一；10例导管上皮不典型增生中，1例为＋＋＋，4例为＋＋，4例为＋，1例为一；20例导管原位癌中，12例为＋＋＋，5例为＋＋，1例为＋，2例为一；FAS在正常乳腺组织、导管上皮不典型增生和导管原位癌中的阳性表达有明显上升趋势，差异有统计学意义（X^2=42．02，P〈0．01）。FAS在乳腺腺病和导管原位癌中的表达也呈升高趋势，差异有统计学意义（X^2=34．69，P〈0．01）。在正常乳腺组织、乳腺腺病、导管上皮不典型增生及导管原位癌中FAS的表达与病变程度呈正相关（X^2=86．02，P〈0．0l；r=0．568，P〈0．01）。在导管原位癌中，FAS的表达与级别无关（x。=9．12，P=0．16）。FAS在5例乳腺浸润性导管癌1级中，4例为＋＋＋，1例为＋’；40例浸润性导管癌2级中，27例为＋＋＋，12例为＋＋，1例为一；15例浸润性导管癌3级中，6例为＋＋＋，5例为＋＋，3例为＋，1例为一；FAS在导管原位癌、浸润性导管癌1级和2级的阳性程度均高于其他病例组，但在3级中FAS表达程度有所下降（X^2=11．26，P=0．01）。FAS在浸润性导管癌中的阳性表达率普遍高�Objective To investigate the expression of fatty acid synthase （FAS） in adenosis, atypical ductal epithelial hyperplasia, ductal carcinoma in situ （DCIS） and invasive ductal carcinoma （IDC） of breast, and the correlation of FAS expression with HER2 gene amplification in IDC. Methods Immunohistochemical EnVision method staining for FAS was performed in 100 cases of breast lesions and 10 normal breast tissues. HER2. gene amplification was detected with FISH in 60 cases of IDC. Results The cohort included 10 cases of adenosis, 10 atypical ductal epithelial hyperplasia, 20 DCIS （8 high-grade,9 intermediated-grade and 3 low-grade）, and 60 cases of IDC （5 grade 1,40 grade 2 and 15 grade 3）. FAS expression was negative in all 10 normal breast tissues; in the 10 cases of adenosis, strongly positive FAS expression was detected in one case, positive in 2, weakly positive in 4, and negative in 3 ; in the 10 cases of atypical ductal epithelial hyperplasia, FAS immunohistochemistry showed that 1 was strongly positive, 4 positive, 4 weakly positive, and 1 negative; in the 20 cases of DCIS, FAS immunostaining showed that 12 were strongly positive, 5 positive, 1 weakly positive, and 2 negative; FAS expression showed a clear increasing trend from normal breast tissue, atypical ductal epithelial hyperplasia to DCIS （ X^2 = 42. 02, P 〈 0. 01 ）. Likewise, the increasing trend was also demonstrated from adenosis to DCIS （ X^2 = 34. 69, P 〈 0. 01 ）. There was also a positive correlation between FAS expression and extent of lesion among normal breast tissue, adenosis, atypical ductal epithelial hyperplasia and DCIS （ ~2 = 86.02, P 〈 0. 01 ; r = O. 568, P 〈 0. 01 ）. FAS expression was not correlated with the grade of DCIS （ ~2 = 9. 12, P = 0. 16）. In the five cases of grade 1 IDC, FAS immunostaining showed that 4 cases were strongly positive and 1 positive ; in the 40 cases of grade 2 IDC, FAS immunostaining showed that 27 strongly positive, 12 positive, and 1 negative; in the 15 cases of grad

关 键 词：乳腺肿瘤 脂肪酸合成酶复合物 基因 ERBB-2 原位杂交 荧光 

分 类 号：R737.9[医药卫生—肿瘤]