干扰素诱导蛋白16对人脑血管外膜成纤维细胞增殖与迁移的影响及其机制  

Effects of interferon-inducible protein 16 on proliferation and migration of human brain vascular adventitial fibroblasts

作　　者：黄晶[1] 宋方[1] 龙向淑[1] 吴强[1] 

机构地区：[1]贵州省人民医院心内科,贵州贵阳550002

出　　处：《中国病理生理杂志》2013年第4期584-589,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81260030);贵州省高层次人才科研条件特助经费项目合同(No.TZJF-2010年-098号);贵州省科技攻关项目(黔科合LS字[2012]021号)

摘　　要：目的:研究干扰素诱导蛋白16(IFI16)对人脑血管外膜成纤维细胞(HBVAFs)增殖与迁移的影响及其可能机制。方法:在HBVAFs中转染针对IFI16基因的小分子干扰RNA(siRNA)48 h后,用2×106U/L干扰素-α(IFN-α)处理转染IFI16 siRNA细胞24 h。流式细胞术测定细胞周期,细胞划线法与Transwell法测定细胞迁移能力。应用real-time PCR法和蛋白免疫印迹(Western blotting)法分别测定细胞中IFI16、p53及p21 mRNA和蛋白表达水平的变化。结果:转染IFI16 siRNA后,HBVAFs中IFI16、p53及p21 mRNA和蛋白表达水平下调,增加了细胞G1/S期转换。IFN-α可诱导HBVAFs中IFI16、p53及p21mRNA和蛋白表达水平上调,同时抑制细胞G1/S期转换与细胞迁移,但在转染了IFI16 siRNA的HBVAFs中IFN-α的上述作用受到抑制。结论:IFI16表达可以抑制HBVAFs增殖和迁移,其机制可能与激活p53和p21的表达有关。AIM： To investigate the effects of interferon-inducible protein 16 （IFI16） on the proliferation and migration of human brain vascular adventitial fibroblasts （HBVAFs）. METHODS： The siRNA of IFI16 gene was trans- fected into HBVAFs. Forty-eight hours after transfection, the cells were exposed to 2 x 106 U/L interferon alpha （IFN-α） for 24 h. Cell cycle was analyzed by flow cytometry. Cell migration was determined by scratch assay and transwell method. The mRNA and protein levels of IFI16, p53 and p21 were measured by real-time PCR and Western blotting, respectively. RESULTS： After transfection with IFI16 siRNA, the expression of IFI16, p53 and p21 at mRNA and protein levels was decreased in HBVAFs, and the cell cycle at G1/S transition was promoted. Meanwhile, stimulated with IFN-ot up-regulated the expression of IFI16, p53 and p21 at mRNA and protein levels, and inhibited the cell cycle transition at G1/S and cell migration in HBVAFs. Such effect was restrained by transfection with IFI16 siRNA into HBVAFs. CONCLUSION： The expression of IFI16 inhibits the proliferation and migration of HBVAFs, which may be related to the activation of p53 and p21 expression.

关 键 词：干扰素诱导蛋白 血管外膜成纤维细胞 细胞增殖 细胞迁移 

分 类 号：R363[医药卫生—病理学]