麦冬抑制大鼠心肌成纤维细胞胶原合成的作用及其机制  

Inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblasts

作　　者：王国钦 李瑞满[2] 金伟 宋国良 陈勤 裴兆辉 

机构地区：[1]南昌市第三医院中医科,江西南昌330009 [2]暨南大学附属第一医院妇产科,广东广州510632 [3]南昌市第三医院心内科,江西南昌330009

出　　处：《中国病理生理杂志》2013年第4期615-618,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.200025370)

摘　　要：目的:观察麦冬抑制大鼠心肌成纤维细胞胶原合成的作用及其机制。方法:以培养的SD大鼠心肌成纤维细胞为观察对象,随机将心肌成纤维细胞分为4组(n=10):对照组(未用麦冬处理大鼠心肌成纤维细胞)、麦冬(10μg/L)组、麦冬(20μg/L)组和麦冬(30μg/L)组。测定不同组心肌成纤维细胞活力、[3H]-脯氨酸掺入率、TGF-β1、p-Smad2/3和Smad2/3蛋白表达的变化。结果:与对照组比较,麦冬(10μg/L)组大鼠心肌成纤维细胞活力、[3H]-脯氨酸掺入率、TGF-β1、p-Smad2/3和Smad2/3表达显著降低(P<0.01)。与麦冬(10μg/L)组比较,麦冬(20μg/L)组大鼠心肌成纤维细胞活力、[3H]-脯氨酸掺入率、TGF-β1、p-Smad2/3和Smad2/3表达显著降低(P<0.01)。与麦冬(20μg/L)组比较,麦冬(30μg/L)组大鼠心肌成纤维细胞活力、[3H]-脯氨酸掺入率、TGF-β1、p-Smad2/3和Smad2/3表达显著降低(P<0.01)。结论:麦冬对大鼠心肌纤维化有明显抑制作用,其机制可能与TGF-β1、p-Smad2/3和Smad2/3蛋白表达的降低有关。[ ABSTRACT] AIM： To investigate the inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblast （CFs） and the underlying mechanism. METHODS： Cultured CFs from Sprague-Dawley （SD） rats were randomized into 4 groups： control group （normal rat cardiac fibroblasts）, Ophiopogon japonicus of 10 μg/L group, Ophiopogon japonicus of 20 μg/L group and Ophiopogonjaponicus of 30 μg/L group. Cell vitality, [SH]-proline incorporation, and the protein ex- pression of TGF-β1-Smad2/3 and total Smad2/3 in CFs were determined. RESULTS： Compared with control, the cell vitality, [ 3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were signifi- cantly decreased in Ophiopogon japonicus of 10 ug/L group. Compared with Ophiopogon japonicus of 10 ug/L group, the cell vitality, [ 3 HI-proline incorporation, and the protein expression of TGF-β1 , p-Smad2/3 and total Smad2/3 were signif- icantly decreased in Ophiopogon japonicus of 20 p^g/L group. Compared with Ophiopogon japonicus of 20 ~g/L group, the cell vitality, [ 3H ]-proline incorporation, and the protein expression of TGF-I3~ , p-Smad2/3 and total Smad2/3 were signifi- cantly decreased in Ophiopogon japonicus of 30 ug/L group. CONCLUSION： Ophiopogon japonicus may inhibit CFs. These actions are related to the changes of [ 3 H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3.

关 键 词：麦冬 心肌成纤维细胞 SMAD2 3 转化生长因子Β 

分 类 号：R363[医药卫生—病理学]