黄芪多糖通过活化AMPK和促进骨骼肌FAT/CD36转位改善成肌细胞FFAs代谢  

APS improves free fatty acid metabolism by activating AMPK and promoting translocation of FAT/CD36 in C2C12 myoblasts

作　　者：胡阳黔[1] 李静[2] 刘坚[2] 欧阳静萍[3] 宋杰[1] 

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442000 [2]湖北医药学院,湖北十堰442000 [3]武汉大学医学院,湖北武汉430079

出　　处：《中国病理生理杂志》2013年第4期637-640,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370673;No.30770734);十堰市科技局科技项目(No.ZD2012037)

摘　　要：目的:探讨黄芪多糖(Astragalus polysaccharides,APS)对骨骼肌游离脂肪酸(free fatty acids,FFAs)代谢的影响及其机制。方法:培养小鼠C2C12成肌细胞;MTT法检测不同浓度FFAs作用不同时间对细胞活性的影响。根据MTT结果选取FFAs最适浓度和时间处理细胞并用APS干预,采用乙酰辅酶A合成酶-乙酰辅酶A氧化酶法检测APS干预前后培养液FFAs浓度;Western blotting测APS干预前后细胞膜脂肪酸转位酶(FAT/CD36)、总FAT/CD36、磷酸化腺苷酸活化蛋白激酶(phosphorylated AMP-activated protein kinase,p-AMPK)和总AMPK蛋白表达。结果:FFAs对细胞的毒性呈浓度和时间依赖性。与FFAs组比较,FFAs+APS组细胞膜FAT/CD36及p-AMPK蛋白表达增加(P<0.05),而总FAT/CD36及总AMPK蛋白表达无明显差异(P>0.05),同时培养液FFAs浓度降低,细胞活性增加(P<0.05)。结论:APS可以增加骨骼肌细胞对FFAs的摄取利用,其机制可能与活化AMPK和促进FAT/CD36转位有关。AIM： To investigate the effect of Astragalus polysaccharides （APS） on the metabolism of free fatty acids （FFAs） in C2C12 myoblasts. METHODS： Cultured C2C12 myoblasts were used in the study. The viability of C,2C12 myoblasts treated with FFAs at different concentrations for different time was observed by MTr assay. The concen- trations of FFAs in the medium were detected by acetyl-CoA synthase （ACS） -acetyl-CoA oxidase （ACOD） method. The expression of fatty acid translocase （ FAT/CD36）, AMPK and p-AMPK protein was examined by Western blotting. RE- SULTS： FFAs decreased the viability of C2C12 myoblasts in a time- and concentration-dependent manner. Compared with FFAs group, the expression of cellular membrane FAT/CD36 and p-AMPK proteins increased in FFAs ＋ APS group, but total AMPK and FAT/CD36 protein expression was not significantly changed. Meanwhile, the concentration of FFAs in the medium decreased and the cell viability increased in FFAs ＋ APS group as compared with the group. CONCLUSION： APS improves the metabolism of FFAs by activating AMPK and promoting translocation of FAT/CD36 in C2C12 myoblasts.

关 键 词：黄芪多糖 游离脂肪酸 腺苷酸活化蛋白激酶 

分 类 号：R363[医药卫生—病理学]