白藜芦醇对高脂饮食去卵巢肥胖大鼠海马组织脑源性神经营养因子水平的影响  

Effect of resveratrol on level of brain-derived neurotrophic factor in hippocampus of obese rats induced by ovariectomy and high-fat diet

作　　者：苗玉连[1] 武传龙[1] 刘金波[1] 江蓓[2] 张晓黎[1] 

机构地区：[1]山东大学齐鲁医院内分泌科,山东济南250012 [2]山东大学齐鲁医院肾内科,山东济南250012

出　　处：《中国病理生理杂志》2013年第4期670-675,共6页Chinese Journal of Pathophysiology

基　　金：山东省优秀中青年科学家科研奖励基金资助项目(No.BS2009YY038);山东大学自主创新基金资助项目(No.2012TS162);山东大学自主创新基金资助项目(No.2012TS204)

摘　　要：目的:探讨去卵巢联合高脂饮食诱导的肥胖大鼠海马组织中脑源性神经营养因子(BDNF)、雌激素受体α(ERα)和雌激素受体β(ERβ)表达的变化,同时观察白藜芦醇对这些改变的影响。方法:50只3月龄雌性Wistar大鼠随机分为5组:假手术普通饮食对照(C)组、假手术高脂饮食(H)组、单纯去卵巢(O)组、去卵巢高脂饮食(O+H)组和白藜芦醇(40 mg.kg-1.d-1)+去卵巢高脂饮食(O+H+R)组。3月后抽取股动脉血检测血清雌二醇(E2)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量;实时荧光定量PCR法检测海马BDNF、ERα和ERβmRNA表达,蛋白免疫印迹法及ELISA法分别检测海马BD-NF蛋白含量。结果:与C组比较,H组大鼠血清TC和LDL-C含量升高,海马BDNF水平显著降低(P<0.05或P<0.01),O组大鼠血清E2水平降低,TC含量升高,海马BDNF水平及ERα、ERβmRNA表达均明显下降(P<0.05或P<0.01);O+H组大鼠血清TC含量升高,HDL-C水平降低,海马BDNF水平降低,与C组、H组和O组比较均有显著差异(P<0.05或P<0.01),海马ERα和ERβmRNA表达下降,与C组和H组比较差异显著(P<0.05或P<0.01);O+H+R组大鼠血清E2水平升高,TC含量降低,海马ERα和ERβmRNA表达水平及BDNF含量均明显增加,与O+H组比较有显著差异(P<0.05或P<0.01)。结论:去卵巢联合高脂饮食显著降低大鼠海马ERα和ERβmRNA表达及BDNF水平,而白藜芦醇能够明显改善绝经后肥胖大鼠的血脂水平,上调海马ERα和ERβmR-NA表达,增加海马BDNF水平。AIM： To explore the effects of resveratrol on the level of brain-derived neurotrophic factor （ BD- NF） and the mRNA expression of estrogen receptor et （ERa） and estrogen receptor 13 （ERI~） in hippocampus of obese rats induced by ovariectomy and high-fat diet. METHODS： Fifty female Wistar rats, aged 3 months, were randomly divided into 5 groups： control （C） group, sham operation plus high-fat diet （H） group, ovariectomy plus normal diet （O） group, ovariectomy plus high-fat diet （ O ＋ H） group, and ovariectomy plus high-fat diet and treated with resveratrol （40 mg~ kg-1 ~ d-1） （O ＋ H ＋ R） group. Three months later, the blood was collected from the femoral artery to detect the serum concentrations of total cholesterol （ TC）, triglyceride （ TG）, high-density lipoprotein cholesterol （ HDL-C ）, low-density lipoprotein cholesterol （LDL-C） and estradiol （ E2 ）. The mRNA expression of ERa, ERI3 and BDNF in the hippocampus was determined by real-time PCR. The protein level of BDNF in hippocampal tissues was detected by ELISA and Western blotting. RESULTS： Compared with C group, the serum levels of TC and LDL-C in H group were increased, and the hip- pocampal level of BDNF was decreased. The rats in O group had higher concentration of serum TC, and lower levels of ser- um E2 and the mRNA expression of ERa, ERI3 and BDNF in the hippocampus than those in C group. Compared with C, H and O groups, the level of serum TC was higher, and the level of serum E2 and the expression of BDNF in the hippocampus was lower in O ＋ H group. The mRNA expression of ERa and ERI3 in hippocampus was also reduced as compared with C group and H group. After treated with resveratrol, the rats in O ＋ H ＋ R group showed lower level of serum TC, and higher levels of serum E2, hippocampal BDNF and mRNA expression of ERa and ERI~ than those in O ＋ H group. CONCLU- SION ： Ovariectomy combined with high-fat diet decreases the mRNA expression of ERctand ERI3 and the level of BDN

关 键 词：卵巢切除术 高脂饮食 肥胖 海马 脑源性神经营养因子 

分 类 号：R285[医药卫生—中药学]