急性髓系白血病中eIF4E高表达与疾病进展相关  

Overexpression of eIF4EGene in Acute Myeloid Leukemia and Its Relation with Disease Progression

作　　者：蒋引娣[1] 卢育洪[1] 陈少华 朱康儿[1] 张学利[1] 郁志[1] 钟隽[1] 张涛[1] 罗更新[1] 陈洁[1] 潘焕玉[1] 李琰[1] 秦恋岸[1] 李扬秋[2,3] 

机构地区：[1]暨南大学附属第一医院血液科 [2]医学院血液病研究所 [3]再生医学教育部重点实验室,广东广州510632

出　　处：《中国实验血液学杂志》2013年第2期296-299,共4页Journal of Experimental Hematology

摘　　要：本研究检测未治疗、未缓解/复发及缓解期间急性髓系白血病(AML)和其他良性血液病患者骨髓细胞中eIF4E基因表达水平并分析其与疾病进展的关系。采用SYBR Green I荧光定量PCR和相对定量分析法检测30例AML患者(M2型6例,M3型5例,M4型8例,M5型10例,M6型1例),11例AML化疗后完全缓解患者和20例非肿瘤性血液病患者骨髓单个核细胞的eIF4E表达情况,以β2微球蛋白基因(β2M)作为内参,采用公式2-△Ct×100%计算eIF4E基因表达水平。结果表明:AML未治疗及未缓解/复发组患者骨髓细胞eIF4E表达水平(7.098±5.544)%明显高于非肿瘤患者(0.248±0.163)%(P<0.01)和化疗后完全缓解患者(0.964±0.312)%(P<0.01),各AML亚型之间不存在统计学差异,尽管M4、M5亚型的表达水平相对较高。非白血病患者与缓解患者比较,eIF4E表达水平不存在显著性差异。结论:eIF4E基因在急性髓系白血病细胞中出现高表达,并随着疾病的缓解而明显降低,因此eIF4E有可能作为监测白血病进展的一项参数。The aim of this study was to detect the expression level of eIF4E gene in patients with non-treated, remis- sion and non-remission/relapse acute myeloid leukemia（ AML）, and other non-malignant haematologic diseases so as to analyze and reveal the relationship of eIF4E gene expression with AML progression. SYBR Green I RT-PCR was used to assay the expression level of eIF4E mRNA extracted from bone marrow mononuclear cells in 30 patients with AML （6 in M2, 5 in M3,8 in M4,10 in M5,1 in M6） and 20 patients with non-malignant hematologic diseases. The β2-microglu- bin（β2M） was used as internal reference and the formula 2^-△Ct×100% was applied to calculate the expression level of eIF4E gene. The results showed that the eIF4E expression level （ 7. 098 ± 5. 544 ） % in patients with non-treated and non-remitted/relapsed AML was significantly higher than that in patients with remission （0. 964± 0. 312）% （P 〈 0.01 ） and non-malignant hematologic diseases （ 0. 248 ± 0. 163 ） % （ P 〈 0.01 ）. There was no difference between latter two group patients, even though the expression level of eIF4E gene in patients with M4 and M5 was higher. As compared with non-malignant hematologic diseases, the expression level of eIF4E gene of patients with remission patients showed no significant difference. It is concluded that the over-expression of eIF4E gene has been found in patients with AML, and its level obviously decreases along with remission of disease, thus the eIF4E gene may be a surveillance parameter for disease progression.

关 键 词：eIF4E基因 实时定量PCR 急性髓系白血病 

分 类 号：R733.7[医药卫生—肿瘤]