板栗花粉黄酮的精制及其质谱分析  

Refinement and MS Analysis of Flavonoids in Chestnut Pollen

作　　者：郭丽梅[1] 王盼盼[1] 

机构地区：[1]天津科技大学材料科学与化学工程学院,天津300457

出　　处：《天津科技大学学报》2013年第2期15-18,共4页Journal of Tianjin University of Science & Technology

摘　　要：对板栗花粉中总黄酮进行提取.用AB–8大孔树脂对总黄酮进行初步分离纯化,其较佳洗脱程序为用200,mL蒸馏水淋洗后使用140,mL 70%乙醇淋洗.粗提液反复纯化后用液相色谱–质谱联用仪对黄酮进行定性分析.结果表明,经过大孔树脂纯化后总黄酮的含量由13.39%提高到78.12%,依据质谱图推断出板栗花粉中黄酮的主要成分为山奈酚、槲皮素、异鼠李素以及杨梅黄酮.Flavonoids in chestnut pollen were extracted by using ethanol. The crude extraction was purified with macropor- ous resin AB-8 using 200 mL distilled water and 140 mL 70% ethanol. The content of flavonoids after refinement increased from 13.39% to 78.12%. The flavonoids from chestnut pollen were analyzed with HPLC-MS. The result showed that there were four kinds of flavonoids in chestnut pollen, which were myricetin, quercetin, kaempferol and isorhamnetin.

关 键 词：板栗花粉 黄酮 精制 质谱 

分 类 号：TQ911[化学工程]