大豆提取液中大豆黄素的紫外分光光度测定法  

UV Spectrometry Method to Determine Daidzein in Soybean Extract

作　　者：蒋盼[1] 顾建明[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《食品研究与开发》2013年第4期77-81,共5页Food Research and Development

摘　　要：紫外分光光度法(248 nm)测定大豆提取液中的大豆黄素浓度与HPLC法测得的值有显著差异(t检验法)。以HPLC法测得的值为可信值,用函数的方法对紫外法标准曲线进行矫正,矫正后的紫外法标准曲线方程为y=0.706 1x+0.012 5,回收率为99.128%,精密度为5.387%,用此方程测得的样液中大豆黄素浓度与HPLC法测得的值无显著差异(t检验法)。There was significant difference between the two daidzein values in the soybean extract determined by UV （at 248 nm）and HPLC （t test, 〉t0.01）. UV standard curve was corrected by mathematic method and HPLC values as truth-values,a new relationship between concentration （y）and absorbency （x）,y=0.706 1x＋0.012 5, was established,with no significant difference between UV and HPLC, the recovery of 99.128 % and the relative standard deviation （RSD）of 5.387 % for daidzein detection.

关 键 词：大豆异黄酮 大豆黄素 测定 紫外分光光度法 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.32[轻工技术与工程—食品科学与工程]